SDF-1 growth stimulation on feline mammary carcinoma primary cultures. A. Scatterplot represents the individual and mean values of increase of growth rate in 6 primary cultures stimulated with SDF-1 (25 nM) or 10% FCS for 24 h, evaluated by MTT assay. *P < 0.05, **P < 0.01 (vs. serum-starved control). B. Dose-response curve of SDF-1 treatment for 24 h. *P < 0.05, **P < 0.01 (vs. untreated serum- starved control) evaluated by MTT assay. C. Effects of CXC4 inhibition by the antagonist AMD3100 on SDF-1 cell growth stimulation evaluated by MTT assay. Cells were treated with SDF-1 (25 nM) for 24 h in the absence or presence of AMD3100 (10 μM). AMD3100 significantly reverts SDF-1 growth stimulus and affects basal proliferation. **P < 0.01 (vs. untreated serum- starved control).