Figure 3

Analysis pSTAT3, STAT3 and survivin in canine OS cell lines following LLL12 treatment. Canine OS cell lines were treated with DMSO or LLL12 for 4, 8 and 12 hours prior to collection. Normal canine osteoblasts were treated with DMSO or LLL12 for 12 hours prior to collection. Protein lysates were generated and separated by SDS-PAGE and Western blotting for pSTAT3, STAT3, survivin and β-actin were performed. Experiments were repeated two times.