Table 2 Cell surface neurogenic markers analysed by RT-PCR
Genes | Accession number | Primers sequence | Size (bp) |
|---|---|---|---|
Nestin | AB257750 | forward 5`- AGACTTCCCTCAGCTTTCAGG-3` | 209 |
reverse 5`-GCCTGGAGGAATTCTTGGTT-3` | |||
N200 | NM174121 | forward 5`TAGCACATTTGCAGGAAGCA-3` | 208 |
reverse 5`-CGGCCAATTCCTCTGTAATG-3` | |||
β-tubulin III | NM001077127 | forward 5`- AGATTCCGCTATTAGCTA-3` | 207 |
reverse 5`- CGGTACCGGTTAATAGTAGT-3 | |||
GFAP | L19867 | forward 5`- TGCAGACCTGACAGACGCTGTTG-3` | 209 |
reverse 5`-CTGCTAGAGGGCGAGGAGAACG-3` | |||
NT3 | NM001077988 | forward 5`- ATGCCGTAGCGGTTAATGCA-3` | 208 |
forward 5`- ATGCCGTAGCGGTTAATGCA-3` | |||
H2A | AW461431 | forward 3`-GTCTTGGAGTACCTGACCGC-5` | 209 |
reverse 3`-AGTCTTCTTCGGGAGCAACA-5` |