Figure 1

Gene expression of NLRC5 , IFNA , IFNB , IL-6 , and MHC class I after LPS treatment in negative siRNA transfection controls. HD11 macrophages were stimulated for 2, 4, 6 and 8 hours with 1 μg/ml LPS. Data are shown as the fold change in mRNA expression between LPS treatment and vehicle-treatment for each gene. a: NLRC5; b: IFNA; c: IFNB; d: IL-6; e: MHC class I; RNA samples were assayed in triplicates by qPCR. P values are flagged with asterisks when lower than 0.05(*) or 0.01 (**).