Figure 1
From: O6-methylguanine-DNA methyltransferase in equine sarcoids: molecular and epigenetic analysis
Illustration of representative immunohistochemistry in equine normal skin and sarcoid lesions. A) MGMT negative immunoreactivity in a sarcoid sample. Neoplastic fibroblasts are negative for MGMT staining. Strepatavidin-biotin peroxidase method. Mayer’s haematoxylin nuclear counterstaining. x 240. B) Sarcoid sample showing very weak MGMT immunoreactivity. Some fibroblasts from the neoplastic tissue show a very faint cytoplasmic immunostaining for MGMT. Strepatavidin-biotin peroxidase method. Mayer’s haematoxylin nuclear counterstaining. IHC. x 240. C) MGMT staining of normal equine skin. Normal fibroblasts from derma displayed nuclear and cytoplasmic staining for MGMT. The different epidermal layers are also stained. Strepatavidin-biotin peroxidase method. Mayer’s haematoxylin nuclear counterstaining. IHC. x 240. D) Human tonsil tissue section. Epithelial cells show positive MGMT immunostaining. Strepatavidin-biotin peroxidase method. Mayer’s haematoxylin nuclear counterstaining. IHC. x 120.