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Figure 3 | BMC Veterinary Research

Figure 3

From: Dolphin Morbillivirus and Toxoplasma gondii coinfection in a Mediterranean fin whale (Balaenoptera physalus)

Figure 3

Molecular and immunohistochemical studies confirmed the presence of T. gondii in the whale's tissues. PCR techniques amplifying a conserved region of coccidian parasites yielded positive results for T. gondii in the heart (lane 1), skeletal muscle (lane 2), mesenteric lymph node (lane 3), and kidney (lane 4); positive and negative controls (C + and C-, respectively) are also shown in figure A along with the DNA ladder marker. IHC by means of a commercially available goat polyclonal anti-T. gondii antiserum solution (1:1000; VMRD Inc, Pullman, WA) showed rare T. gondii cysts embedded into both the myocardial (B; 400×, bar = 20 μm) and the renal tissue.

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