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Table 1 Pens of cattle sampled in Alberta Canada in 2007 with 1 fecal grab sample and 1 hide swab collected per steer at each sampling and PFGE performed on samples positive for E. coli O157:H7

From: Genetic diversity and antimicrobial resistance among isolates of Escherichia coliO157: H7 from feces and hides of super-shedders and low-shedding pen-mates in two commercial beef feedlots

Feedlot A B Total
Pen 1 2 3 4 5 6 7 1 2 3 4  
cattle (n) 153 152 153 253 258 155 233 151 235 138 106 1987
Sample 1 date May 22 Jun 4 Jul 20 Jul 27 Sep 26 Oct 9 Oct 23 Jul 6 Jul 12 Aug 10 Aug 30  
  SS fecal isolates (n) 2 36 12 19 7 3 0 19x 44 10 1 153
  SS perineal swabs (n) 2 30 11 17 3 3 0 17 40 7 1 131
  LS fecal isolates (n) 13 37 100 54 24 2 1 36 107 20 31 425
Sample 2 date Sep 24 Sep 6 Oct 3 Oct 17 Dec 10 Nov 29 Dec 6 Sep 28 Sep 28 Nov 14 Nov 27  
SS fecal isolates (n) 1 0 0 0 0 0 0 8x 0 0 0 9
  SS perineal swabs (n) 0 0 0 0 0 0 0 6 0 0 0 6
  LS fecal isolates (n) 3 10 0 2 3 0 1 40 9 3 0 71
  LS fecal isolates from former SS (n) 0 1 0 2 2 0 0 7 9 0 0 21
Total isolates tested by PFGE (n) 21 113 123 92 37 8 2 126 200 40 33 795
Total REPC (n) 1 12 14 14 6 1 1 20 13 6 6 85y
Total unique isolates (n) 8 33 11 14 12 6 0 13 17 8 11 86y
SS fecal & SS perineal swab in same REPC (n) 1 1 0 0 0 0 NAz 0 0 0 0 2
  1. Numbers of super–shedders (SS)v and low- shedders (LS)w within pens. Numbers of, restriction endonuclease digestion clusters (REPC, 90% or greater similarity) and unique isolates (< 90% similarity) within and across pens.
  2. vSuper-shedder, shedding at least 104 CFU E. coli O157:H7/g feces.
  3. wLow-shedder, shedding < 104 CFU E. coli O157:H7/g feces.
  4. xOne steer was a super-shedder at both samplings.
  5. yTotal comparing all isolates across pens.
  6. zNA, not applicable, no super-shedders in pen.