BLV-CoCoMo-qPCR: a useful tool for evaluating bovine leukemia virus infection status

Jimba, Mayuko; Takeshima, Shin-nosuke; Murakami, Hironobu; Kohara, Junko; Kobayashi, Naohiko; Matsuhashi, Tamako; Ohmori, Takashi; Nunoya, Tetsuo; Aida, Yoko

doi:10.1186/1746-6148-8-167

BMC Veterinary Research

Table 2 Comparison of BLV detection methods using 370 cattle

From: BLV-CoCoMo-qPCR: a useful tool for evaluating bovine leukemia virus infection status

(a) Copy number/10⁵ cells by BLV-CoCoMo-qPCR=0
Animal no.	Copy number^a	Nested PCR	PHA^b	AGID^c	ELISA^d
1-39	0	-^e	-	-	-
40-53	0	-	+^f	-	-
54-85	0	-	-	-	+
86	0	-	-	+	-
87-123	0	-	+	-	+
124-129	0	-	-	+	+
130-138	0	-	+	+	+
139-141	0	-	-	+	NT^g
142	0	-	NT	-	-
143	0	-	NT	-	+
144	0	-	NT	-	NT
145-148	0	-	+	-	NT
149,150	0	-	+	+	NT
151,152	0	NT	-	-	+
153	0	NT	-	+	+
154	0	NT	+	-	-
155	0	NT	+	+	NT
156	0	NT	+	-	NT
157-159	0	NT	+	-	+
160,161	0	NT	+	+	+
162	0	+	-	-	+
163	0	+	+	-	-
-	163	150	85	138	57
+	0	2	75	25	94
Tested	163	152	160	163	151
(b) Copy number/105 cells by BLV-CoCoMo-qPCR>0
164	1	-	+	+	+
165	1	+	+	-	+
166	1	-	-	-	-
167	2	+	-	+	+
168	3	+	+	+	+
169	3	NT	+	-	+
170	4	+	-	+	+
171	5	-	+	-	-
172	6	+	+	+	NT
173	8	+	+	-	+
174	9	-	+	-	+
175	9	+	-	-	+
176	9	NT	+	+	NT
177	10	+	-	+	+
178	12	+	+	+	+
179	15	+	-	+	NT
180,181	16,17	+	-	+	+
182	17	+	+	+	+
183	17	NT	+	+	+
184	18	-	+	+	+
185	19	+	-	+	+
186,187	20,21	-	+	+	+
188	22	+	-	+	+
189	23	-	-	-	+
190	25	+	+	+	+
191	27	-	-	+	+
192	33	+	-	+	+
193	37	-	-	+	+
194	39	-	+	+	+
195	40	-	+	+	NT
196	41	+	-	+	+
197	42	+	+	+	+
198,199	43,44	+	-	+	+
200	45	-	+	-	+
201	49	+	+	+	+
202	50	-	+	+	+
203	53	+	+	+	+
204	55	NT	+	+	+
205	58	-	+	+	+
206	67	+	+	+	+
207	68	NT	-	+	+
208–210	71,74,79	+	+	+	+
211	83	-	NT	+	+
212	85	+	-	+	+
213	91	NT	-	+	+
214,215	92,96	+	+	+	NT
216	99	-	-	+	+
217	110	+	+	-	+
218	110	-	-	+	+
219	110	+	+	+	NT
220	145	+	+	-	+
221	145	+	+	+	+
222	149	-	-	+	+
223	154	+	NT	+	NT
224	166	+	+	+	+
225	166	+	-	+	NT
226	176	NT	-	+	+
227	176	+	+	-	+
228	180	-	+	+	NT
229,230	190,194	+	+	+	NT
231	202	NT	+	+	+
232	203	+	+	+	+
233	231	+	-	-	+
234	254	+	+	+	+
235	261	+	+	-	+
236	265	+	NT	+	+
237	270	+	+	+	+
238	295	+	-	+	+
239	321	+	+	+	NT
240	347	+	+	+	+
241	349	NT	+	+	+
242,243	363,366	+	+	+	+
244	374	+	-	+	+
245	472	+	+	-	+
246	475	+	+	+	NT
247	491	+	+	-	+
248	565	-	-	+	+
249	605	+	+	+	NT
250	634	-	+	+	+
251,252	664,684	+	-	+	+
253,254	705,710	+	+	+	+
255-257	835,868,875	+	-	+	+
258	876	+	+	+	+
259	942	+	-	+	NT
260,261	1088,1170	+	-	+	+
262	1196	+	+	+	NT
263	1219	+	-	+	+
264,265	1223,1225	+	+	+	+
266	1233	-	-	-	NT
267,268	1279,1282	+	-	+	+
269	1445	+	NT	+	+
270	1459	+	+	+	NT
271	1495	+	-	+	+
272,273	1501,1567	+	+	+	+
274	1648	+	-	+	+
275	1671	+	+	+	+
276	1793	+	+	+	NT
277	1844	+	-	+	NT
278	1902	+	+	+	NT
279	2068	+	-	+	+
280-282	2130,2142, 2183	+	+	+	+
283	2218	-	NT	+	+
284	2219	+	-	+	+
285	2326	+	NT	+	+
286	2338	+	+	+	+
287	2524	+	+	-	+
288	2616	+	+	+	NT
289,290	2621,2727	+	+	+	+
291	2849	NT	+	+	+
292	2855	+	-	+	+
293	2963	+	+	+	+
294	3062	+	-	+	+
295,296	3097,3192	+	+	+	+
297	3294	+	+	+	NT
298	3512	NT	+	+	+
299	3619	NT	+	+	NT
300	3833	+	-	+	+
301	3859	NT	-	+	NT
302	3871	+	+	+	+
303	3955	+	+	+	+
304	4291	+	-	+	NT
305	4517	+	+	+	+
306	4623	NT	-	+	+
307,308	4774,4837	+	+	+	+
309,310	5079,5291	+	+	+	NT
311	5296	NT	-	-	NT
312	5453	+	+	+	NT
313	5490	+	-	+	NT
314,315	5551,5667	+	+	-	+
316	5821	+	+	+	+
317,318	6188,6423	+	-	+	NT
319	6471	NT	+	+	+
320	6567	+	+	+	+
321	6686	+	-	+	+
322	7127	+	+	+	+
323	7303	+	-	+	NT
324	7371	+	+	-	+
325	7376	+	NT	+	+
326	7665	+	-	+	+
327	7835	+	+	+	NT
328	7903	+	-	+	+
329	8879	+	+	+	NT
330	9048	+	-	+	+
331,332	9275,9358	+	+	+	+
333	9417	+	-	+	NT
334,335	9843,10154	+	+	+	NT
336	10252	+	+	-	+
337	10363	NT	+	+	+
338	11874	+	-	-	+
339	12040	NT	-	+	NT
340	12461	+	-	+	+
341	12667	+	+	+	NT
342	12791	+	-	+	+
343,344	13136,13229	+	+	+	+
345	13379	+	-	+	+
346	13904	+	+	+	+
347	14057	+	+	+	NT
348	14433	+	+	+	+
349	15602	NT	-	+	+
350	15747	+	-	+	NT
351	15982	+	NT	+	+
352	16099	+	+	+	+
353	16220	NT	+	+	+
354	16984	+	+	+	+
355	17577	+	+	+	NT
356	18419	+	+	+	+
357	18624	NT	+	+	NT
358	19043	NT	+	+	+
359-361	19732,21744 25912	+	+	+	+
362	26883	+	-	+	NT
363	27719	NT	+	+	NT
364	28154	+	+	+	NT
365	28934	+	+	-	+
366	30098	+	-	+	NT
367	32909	+	+	-	+
368,369	36753,42015	+	+	+	NT
370	52680	NT	-	+	NT
-	0	22	72	26	2
+	208	161	127	181	152
Tested	208	183	199	207	154

^aCopy number/10⁵cells by BLV-CoCoMo-qPCR.
^bPHA, Passive hemagglutination antigen method performed with the Bovine Leucosis antibody assay kit “Nisseiken”.
^cAGID, Agarose gel Immuno-diffusiontest was also performed for anti-BLV antibody detection with a commercial bovine leukemia virus antibody test kit.
^dELISA, Enzyme-linked immunosorbent assay was performed with an ELISA kit for detecting anti-BLV antibody.
^e-, Negative.
^f+, Positive.
^gNT, Not test.

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ISSN: 1746-6148

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