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Table 1 Comparison of BLV proviral detection by BLV-CoCoMo-qPCR, the TaqMan MGB assay, and TaKaRa cycleave PCR

From: BLV-CoCoMo-qPCR: a useful tool for evaluating bovine leukemia virus infection status

pBLV-IF

CoCoMo-qPCRa

Lew Methodb

TaKaRa

(copy number)

Cycleave PCRc

100

3/3d

3/3

3/3

50

3/3

3/3

3/3

25

3/3

3/3

3/3

12.5

3/3

3/3

3/3

6.25

3/3

3/3

3/3

3.125

3/3

3/3

3/3

1.5625

3/3

0/3

3/3

0.78125

3/3

0/3

2/3

0

0/3

0/3

0/3

  1. a BLV LTR genes were detected by BLV-CoCoMo-qPCR (Jimba et al., 2010).
  2. b BLV pol gene was detected by TaqMan MGB assay developed by Lew et al. (Lew et al., 2004).
  3. c BLV tax gene was detected by the cycleave PCR BLV detection kit (TaKaRa Bio inc.).
  4. d Number detected per number tested.