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Table 1 List of primers used in amplification and cloning of AIV genes

From: Co-administration of avian influenza virus H5 plasmid DNA with chicken IL-15 and IL-18 enhanced chickens immune responses

Primer name Primer sequence Gene size (bp)
H5fSacII 5’ CCC CCG CGG ATG GAG AAA ATA GTG CTT 3’ H5 gene/1722
H5rAccI 5’ CCC GTC GAC AAT GCA AAT TCT GCA 3’  
IL-15fSacII 5’ AAA CCG CGG ATG CTG GGG ATG GCA CAG 3’ IL-15 gene/564
IL-15rAccI 5’ GGG GTC GAC CTG GTA AGT TGC AAA TCT TGC 3’  
IL-18fSacII 5’ CCC CCG CGG ATG AGC TGT GAA GAG ATC 3’ IL-18 gene/486
IL-18rAccI 5’ CCC GTC GAC AAA GGC CAA GAA CAT TCC TTG 3’