Table 1 List of primers used in amplification and cloning of AIV genes
Primer name | Primer sequence | Gene size (bp) |
|---|---|---|
H5fSacII | 5’ CCC CCG CGG ATG GAG AAA ATA GTG CTT 3’ | H5 gene/1722 |
H5rAccI | 5’ CCC GTC GAC AAT GCA AAT TCT GCA 3’ |  |
IL-15fSacII | 5’ AAA CCG CGG ATG CTG GGG ATG GCA CAG 3’ | IL-15 gene/564 |
IL-15rAccI | 5’ GGG GTC GAC CTG GTA AGT TGC AAA TCT TGC 3’ |  |
IL-18fSacII | 5’ CCC CCG CGG ATG AGC TGT GAA GAG ATC 3’ | IL-18 gene/486 |
IL-18rAccI | 5’ CCC GTC GAC AAA GGC CAA GAA CAT TCC TTG 3’ |  |