Table 2 Primers used in qPCR assays
Genbank acc. | Gene | Primer sequence (5’-3’) | Function | Amplicon (bp) | Primer efficiency |
|---|---|---|---|---|---|
BU694011 | Keratin 14 (Krt14) | F: CAAGGTGGTGATCGTCACAG R: TGGGACCTTAAGAAGCGTGT | Cytoskeleton, cell shape | 83 | 1.91 |
DW572073 | Interleukin 22 (IL22) | F: GGAGAAGCAGGACAAGCATC R: ATAGCACAGCCGTGTTCCTT | Immune | 93 | 1.89 |
209733777 | MHC class I (MHCI) | F: CTCAGTCACGCAAGAGCAAG R: AGCCATGTTTCCACTGAAGG | Immune: antigen presentation | 111 | 2.04 |
BT047112 | E3 ubiquitin-protein ligase LINCR (Lincr) | F: CTGGGGACACCTTCAGACAT R: TACGCATAGCTCCACACCAG | Immune: effector | 114 | 1.89 |
DY692748 | Type-2 ice-structuring protein (AFP2) | F: GGTTGCAGCAGCACCTAAA R: CCGAGGAGTGTTCACAAACAT | Immune: lectin | 100 | 1.90 |
EG833741 | Cytochrome b558 alpha-subunit (p22phox) | F: GGCACCAGCGTAGAAAGAAC R: GCAGATCGCTGCATGTAGAA | Immune: oxidative burst | 111 | 2.02 |
223647763 | Arginase-2, mitochondrial (Arg2) | F: GACAGGCTCGGCATTCAGA R: AAAGACGGGTCCATCGCAT | Immune, amino acid metabolism | 110 | 2.05 |
CA060324 | Annexin A1 (ANXA) | F: GTCAGAATCTTGGTCCTGGTTC R: ACTGCCGTAGTGAAGTGTGCT | Inflammation, exocytosis | 98 | 2.04 |
DW531828 | Cysteine dioxygenase type 1 (CDO1) | F: TCATTGCTCTCGCTCTGCT R: GAGTTATTGCCAATGAGCTTCAG | Metabolism: amino acids | 82 | 2.00 |
DY730337 | Sulfate transporter (Sult) | F: AGGCAAAAGAGATCCCAGGT R: CCCAATGTCAATACCGCTCT | Metabolism: bile, glutathione, xenobiotics | 113 | 2.01 |
CA041487 | Fatty acyl-CoA hydrolase, medium chain (Acot) | F: GGTCCCTCTTCAGGTGTTGA R: TTTGCTCGTACAGGGTCTCC | Metabolism: fatty acids | 121 | 1.97 |
DW533963 | Fatty acid-binding protein, intestinal (FABP2a2) | F: CAGCTACGATGGAGTCGAAGCCA R: GGTTGTAAAATGTTCAGTGTCAC | Metabolism: fatty acids | 139 | 1.96 |
EG934966 | Cytochrome P450 24A1 (CYP24A1) | F: GCGTGTTACCCAGGATGAGT R: GGGAAATTCTCCTCGTCCAT | Metabolism: steroids | 105 | 1.96 |
DY728977 | Cytochrome P450 2 M1 (CYP2M1) | F: TCAGTCCCACCTCTGTACCC R: AATTTGGGATCAGCAAGCA | Metabolism: xenobiotic | 118 | 1.97 |
DW532464 | Aquaporin-8 (Aqp8) | F: GTTGGCATAGTTCTCCTTTGATG R: TTTCAACCCTCCCTTCACC | Water channel | 148 | 1.96 |
AF321836 | Elongation factor 1A (EF1A) | F: GTGCTGTGCTTATCGTTGCT R: GGCTCTGTGGAGTCCATCTT | Translation factor | 148 | 1.91 |
BT050045 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | F: AAGTGAAGCAGGAGGGTGGAA R: CAGCCTCACCCCATTTGATG | Glycolytic enzyme | 96 | 1.89 |
BT043501 | Hypoxanthine phospho-ribosyltransferase 1(HPRTI) | F: CCGCCTCAAGAGCTACTGTAAT R: GTCTGGAACCTCAAACCCTATG | Purine metabolism | 255 | 1.99 |
BG936649 | RNA polymerase II (RNAPOLII) | F:CCAATACATGACCAAATATGAAAGG R: ATGATGATGGGGATCTTCCTGC | DNA transcription | 157 | 1.85 |