Effect of pre-weaning concentrate supplementation on peripheral distribution of leukocytes, functional activity of neutrophils, acute phase protein and behavioural responses of abruptly weaned and housed beef calves

Table 1 Antibodies used in the immunostaining of leukocyte surface markers

Specificity	Cell types	Clone	Isotype	Working solution	Source¹
CD4	T-helper/inducer cells	CC8	IgG₂a	7 μL/mL	Serotec
CD8	T-cytotoxic/suppressor cells	CC63	IgG₂a	7 μL/mL	Serotec
WC1	Subset of γδ T cells	CC15	IgG₂a	7 μL/mL	Serotec
MHC class II	Antigen presenting cells (B cells, activated T cells)	H42A	IgG₂a	7 μL/mL	VMRD
CD62L	L-selectin	BAQ92A	IgG₁	14 μL/mL	VMRD
G1	Neutrophils (and eosinophils)	MM20A	IgG₁	14 μL/mL	VMRD
CD45	All leukocytes (pan marker)²	CC1	IgG₁	3.5 μL/mL	Serotec
IgG₁ isotype	Mouse IgG₁a negative control	-	-	7 μL/mL	Serotec
IgG₂a isotype	Mouse IgG₂a negative control	-	-	7 μL/mL	Serotec

¹Serotec (Oxford, UK), VMRD (Pullman, WA, USA).
²Used to differentiate leukocyte populations and exclude debris (gate analysis).

ISSN: 1746-6148