Identification of Mycobacterium spp. of veterinary importance using rpoB gene sequencing

BMC Veterinary Research

Table 2 Results of PCR assays for five insertion elements for 12 veterinary isolates clustering within the clade containing M. avium subsp. paratuberculosis, M. avium subsp. avium, M. avium subsp. hominissuis, and M. avium subsp. silvaticum.

Isolate ID	Isolate source	IS900	IS901	DT1	IS1245* (long)	IS1245** (short)
10-5560	pig	neg	neg	neg	pos	pos
10-5561	pig	neg	neg	neg	pos	pos
10-5763	pig	neg	neg	neg	pos	pos
09-8107	elk	neg	neg	neg	pos	pos
10-7710	cow	neg	neg	neg	pos	pos
09-4418	cow	neg	neg	neg	pos	pos
09-4995	pig	neg	neg	neg	pos	pos
10-1377	cow	neg	neg	neg	pos	pos
09-4604	cow	neg	neg	neg	pos	pos
09-7368	deer	neg	neg	neg	pos	pos
10-1301	cat	neg	neg	neg	pos	pos
10-0824	cow	neg	pos	neg	neg	neg
M. a. a.		neg	pos	pos	pos	pos
M. a. h.		neg	neg	neg	pos	pos
M. a. p.		pos	neg	neg	neg	neg
M intra.		neg	neg	pos	neg	neg

* 'long' primers from Van Soolingen et al. (33) ** 'short' primers from Johansen et al. (20)
neg = negative, pos = positive
M.a.a: M. avium subsp. avium; M.a.h.: M. avium subsp. hominissuis; M.a.p.: M. avium subsp. paratuberculosis; M. intra.: M. intracellulare

ISSN: 1746-6148