Classical scrapie prions in ovine blood are associated with B lymphocytes and platelet-rich plasma

BMC Veterinary Research

Table 2 Summary of PrP^Sc detection in multiple tissues from sheep transfused with different blood components from scrapie infected sheep.

Blood components	Recipient	PrP^Sc detection by IHC			*Scrapie
	PRNP	Antemortem	Postmortem		positive (%)
		Rectal tissues	Rectal tissues	Other lymphoid tissues
Whole blood	MARQ/MARQ	4/4	4/4	4/4	100
	MARQ/MVRQ	3/4	4/4	4/4	100
	MARQ/TARQ	0/2	0/2	0/2	0
	TARQ/MVRQ	0/3	2/3	3/3	100
Buffy coat	MARQ/MARQ	3/3	3/3	3/3	100
	MARQ/TARQ	1/2	1/2	2/2	100
PBMCs	MVRQ/MVRQ	4/5	5/5	5/5	100
	MARQ/MVRQ	6/9	7/9	9/9	100
	TARQ/MVRQ	1/1	1/1	1/1	100
CD72⁺ B lymphocytes	MVRQ/MVRQ	3/3	3/3	3/3	100
CD21⁺ B lymphocytes	MVRQ/MVRQ	2/3	3/3	3/3	100
Platelet-rich plasma	MARQ/MARQ	2/3	2/3	2/3	67
Platelet-poor plasma	MARQ/MARQ	0/3	0/3	0/3	0

Other lymphoid tissues = retropharyngeal lymph nodes, palatine tonsil, spleen, ileo-cecal junction, ileo-cecal lymph node or mesenteric lymph nodes; IHC = immunohistochemistry; *Scrapie positive PrP^Sc was detected in one or more lymphoid tissues at necropsy

ISSN: 1746-6148