Serological testing of cattle experimentally infected with Mycoplasma mycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns

Table 3 Examination of Panel 1 and 2 sera: Comparison of all serological tests*

Assays compared	Number (percentage) of animals reacting similarly in the tests compared
	30 sera of Panel 1	Selection of 30 sera of Panel 2 (including IBT)	All 178 sera of Panel 2 (without IBT)
cELISA and in-house CFT	21 (70%), ĸ = 0,46^c	18 (60%), ĸ = 0,33^d	114 (70%), ĸ = 0,43^c
cELISA and CIRAD CFT	23 (77%), ĸ = 0,58^c	18 (60%), ĸ = 0,33^d	108 (62%), ĸ = 0,38^d
In-house CFT and CIRAD CFT	27 (90%), ĸ = 0,79^a	30 (100%), ĸ = 1,00^a	161 (90%), ĸ = 0,80^a
In-house CFT and IBT	21 (70%), ĸ = 0,39^d	26 (87%), ĸ = 0,33^d
CIRAD CFT and IBT	21 (70%), ĸ = 0,39^d	26 (87%), ĸ = 0,33^d
cELISA and IBT	17 (57%), ĸ = 0,23^d	19 (63%), ĸ = 0,38^d
In-house CFT, CIRAD CFT and cELISA	21(70%)	18 (60%)	102 (59%)
In-house CFT, CIRAD CFT, cELISA and IBT	15 (45%)	18 (60%)

* Kappa agreement testing [23] was additionally conducted. According to standard criteria, the concordance between two tests compared is classified as ^a very good (1-0.76), ^b good (0.75-0.61), ^c acceptable (0.6-0.4), ^d poor (< 0.4).

ISSN: 1746-6148