Figure 2

Co-localization of PCV1 in the epithelial cells. Co-localization of PCV1 in the epithelial cells of the lungs of a CCL33-inoculated foetus (Bar = 100 μm) and a 3384-inoculated foetus (Bar = 200 μm) collected at 21 days post inoculation (76 days of gestation). Methanol-fixed cryostat sections were incubated (as described in Materials and Methods) with a cytokeratin marker monoclonal antibody AE1/AE3 to stain epithelial cells (green fluorescence). Cells containing PCV1 antigens were localized using biotin-labelled mono-specific porcine anti-PCV1 polyclonal antibodies (red fluorescence).