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Table 1 Specificity testing of rt-PCR assays with isolates of the M.mycoides cluster (A), mollicute strains frequently isolated from bovine lungs (B) and bacterial strains causing CBPP-like symptoms (C)

From: Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoidesSC through experimental infection in cattle

  Species Strain n Host Origin Year MSC_0136 rt-PCR MSC_1046 rt-PCR
A M. mycoides PG1T   cattle ? ? + +
  subsp. Mycoides SC diverse 19 cattle Italy 1990-1992 + +
   diverse 3 cattle Spain unknown + +
   P0   cattle France 1967 + +
   N6   cattle Botswana 1996 + +
   M375   cattle Botswana 1995 + +
   Afade   cattle Cameroon 1968 + +
   PO2   cattle France 1980 + +
   L2   cattle Italy 1993 + +
   Fatick   cattle Senegal 1968 + +
   C11   cattle Chad 1962 + +
   95014   cattle Tanzania 1995 + +
   T1/44   cattle Tanzania 1952 + +
   Gladysdale   cattle Australia 1953 + +
   Shawawa   cattle Botswana 1995 + +
   2091   cattle France 1984 + +
   197   cattle Italy 1992 + +
   B103   cattle Portugal 1986 + +
   6305   cattle Portugal 1993 + +
   6526   cattle Portugal 1993 + +
   Astercous   cattle Spain 1987 + +
   Madrid   cattle Spain 1993 + +
   Tan8   cattle Tanzania 1998 + +
   Matapi   cattle Namibia 2004 + +
   Mandigwan   cattle Namibia 2001 + +
   V5   cattle Australia 1936 + +
   IS31   cattle Tanzania 1998 + +
   2022   cattle France 1984 + +
   B237   cattle Kenya 1987 + +
  M. mycoides PG3, NCTC 10137 T   goat Turkey 1950 - -
  subsp. capri Y-Goat   goat Australia 1956 - -
   7302   goat Portugal < 1994 - -
   Kombolcha   goat Ethiopia 1975 - -
   7730   goat France < 1997 - -
   9096-C9415   goat Nigeria unknown - -
   diverse 6 goat Gran Canaria 1993 - -
   My-I   goat Croatia 1986 - -
   D2482   goat Switzerland 1991 - -
   95010   goat France 1995 - -
   6443-90   goat France 1990 - -
   My-325   goat Croatia 1986 - -
   C260/4   goat Gran Canaria 1993 - -
   Wk354   goat Switzerland 1980 - -
   L   goat France 1975 - -
   Wi18079   goat Germany 2009 - -
   diverse 3 Barbary sheep Germany 1994 - -
   M-5   goat Croatia 1988 - -
   M-18   goat Croatia 1988 - -
   M-29   goat Croatia 1988 - -
  M. capricolum Cal. kid, NCTC 10154 T   goat USA 1954 - -
  subsp. capricolum C47   sheep Germany < 1992 - -
   4146   goat France 1980 - -
   7714   goat France 1967 - -
   609-79   goat France 1979 - -
   8086-1   goat France 1980 - -
  M. capricolum F38, NCTC 10192 T   goat Kenya 1976 - -
  subsp. capripneumoniae 87F05   goat Turkey 2005 - -
   diverse 2 gazelle UAE 2004 - -
   3535   mouflon Qatar 2005 - -
   GL97P   goat Tunisia 1980 - -
  M. leachii PG50, NCTC 10133 T   cattle Australia 1963 - -
   D318b   cattle Germany unknown - -
   D424   cattle Germany unknown - -
   Calf1   cattle Nigeria unknown - -
  M.nov.spec. 8756-13   mountain goat USA 1987 - -
   G1650   capricorn Germany 1994 - -
   G5847   capricorn Germany 1993 - -
B M. putrefaciens KS1, NCTC 10155 T   goat USA 1955 - -
   7578.94   goat France 1994 - -
   Tours2   goat France 1972 - -
  M. bovis PG45, NCTC 10131 T   cattle USA 1962 - -
   363/88   cattle Germany 1988 - -
   334/96   cattle Germany 1996 - -
   54/10   cattle Germany 2010 - -
  M. bovirhinis PG43, NCTC 10118 T   cattle UK 1967 - -
   320/93   cattle Germany 1993 - -
   19/94   cattle Germany 1994 - -
   365/09   cattle Germany 2009 - -
  M. bovigenitalium PG11, NCTC 10122 T   cattle UK 1947 - -
   338/87   cattle Germany 1987 - -
   18/92   cattle Germany 1992 - -
   466/94   cattle Germany 1994 - -
  M. arginini G230, NCTC 10129 T   mouse USA 1968 - -
   162/81   cattle Germany 1981 - -
   208/94   Barbary sheep Germany 1994 - -
   23/05   sheep Germany 2005 - -
  M. californicum St-6, NCTC 10189 T   cattle USA 1981 - -
   456/83   cattle Germany 1983 - -
   111/84   cattle Germany 1984 - -
  M. canadense 275C, NCTC 10152 T   cattle Canada 1974 - -
   380/94   cattle Germany 1994 - -
  M. verecundum 107, NCTC 10145 T   cattle UK 1974 - -
   49/94   cattle Germany 1994 - -
  M. alkalescense PG51, NCTC 10135 T   cattle Australia 1961 - -
   26/93   cattle Germany 1993 - -
  M. canis PG14, NCTC 10146 T   dog UK 1951 - -
   306/93   dog Germany 1993 - -
  A. laidlawii PG8, NCTC 10116 T   unknown UK 1967 - -
   54/99   cattle UK 1999 - -
  A. axanthum S743, NCTC 10138 T   tissue culture UK 1970 - -
   824/80   cattle Germany 1980 - -
  Ureaplasma diversum A417, NCTC 10182 T   cattle UK 1972 - -
C Pasteurella multocida ATCC 43137 T   pig    - -
  Mannheimia haemolytica ATCC 33396 T   unknown    - -