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Figure 4 | BMC Veterinary Research

Figure 4

From: Development of a PCR Assay to detect Papillomavirus Infection in the Snow Leopard

Figure 4

Amplification of the UuPV1 E6 and E7 genes from paired saliva and blood samples. 50 ng of genomic DNA isolated using the Orageneā€¢ANIMAL kit (An), or paired blood samples (Bl) from three snow leopards (SL 1, 2 and 3) were used for PCR amplification. Amplification products were resolved by agarose gel electrophoresis and visualized by staining with SYBRĀ® Green. (a) Amplification of a 694-bp fragment encompassing the E6 viral oncogene from saliva (lanes 3, 5 and 7) and blood (lanes 2, 4 and 6). An amplification control with no DNA was performed in parallel (lane 1). (b) Amplification of a 472-bp DNA fragment encompassing the E7 viral oncogene from paired saliva (lanes 3, 5 and 7) and blood samples (Bl; lanes 2, 4 and 6). Lane 1 shows a control amplification reaction with no DNA. (c) Control amplification reactions of a 503-bp fragment in the coding region the snow leopard Transferrin Receptor (TfR) gene, using paired saliva (An; lanes 2, 4 and 6) and blood samples (Bl; lanes 1, 3 and 5). The negative amplification control with no DNA is shown in lane 7.

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