Figure 4
From: Development of a PCR Assay to detect Papillomavirus Infection in the Snow Leopard
Amplification of the UuPV1 E6 and E7 genes from paired saliva and blood samples. 50 ng of genomic DNA isolated using the Oragene•ANIMAL kit (An), or paired blood samples (Bl) from three snow leopards (SL 1, 2 and 3) were used for PCR amplification. Amplification products were resolved by agarose gel electrophoresis and visualized by staining with SYBR® Green. (a) Amplification of a 694-bp fragment encompassing the E6 viral oncogene from saliva (lanes 3, 5 and 7) and blood (lanes 2, 4 and 6). An amplification control with no DNA was performed in parallel (lane 1). (b) Amplification of a 472-bp DNA fragment encompassing the E7 viral oncogene from paired saliva (lanes 3, 5 and 7) and blood samples (Bl; lanes 2, 4 and 6). Lane 1 shows a control amplification reaction with no DNA. (c) Control amplification reactions of a 503-bp fragment in the coding region the snow leopard Transferrin Receptor (TfR) gene, using paired saliva (An; lanes 2, 4 and 6) and blood samples (Bl; lanes 1, 3 and 5). The negative amplification control with no DNA is shown in lane 7.