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Table 1 RT-PCR primers used to amplify the portions of the ICE sequence

From: A novel integrase-containing element may interact with Laem-Singh virus (LSNV) to cause slow growth in giant tiger shrimp

Primer names Sequence 5'-3' Melting temperature (Tm, °C) Expected product (bp)
Probe 1-Fr   57.56 397
Probe1-Rv   57.80  
Probe 2-Fr   60.40 642
Probe 2-Rv   58.40  
Probe 3-Fr   58.55 399
Probe 3-Rv   58.70  
LSNV-Fr LSNV-Rv   60 61 600
Nested primers
Probe 3-NFr   60 204
Probe 3-NRv   60  
LSNV-NFr   64 195
LSNV-NRv   66  
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