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Figure 4 | BMC Veterinary Research

Figure 4

From: Antibody response to a sterile filtered PPD tuberculin in M. bovis infected and M. bovis sensitized cattle

Figure 4

2D-Western blot analysis of the antibody response to SF-PPD proteins in sera from an M. bovis sensitized bovine. SF-PPD (200 μg) was loaded onto a 17 cm pre-cast acrylamide strip with an immobilized pH range of 3-10 (ReadyStrip™; Bio-Rad). Following isoelectric focussing, the acrylamide strip was loaded into vertical 12% polyacrylamide gels with molecular weight standards (Pre-stained - Low range, Bio-Rad). Protein was electrophoretically transferred to nitrocellulose and blotted with M. bovis sensitized cattle sera (animal # 893).

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BMC Veterinary Research

ISSN: 1746-6148

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