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Table 2 Composition and diplotypes of the USMARC Sheep PRNP Control Panel version 28

From: Ovine reference materials and assays for prion genetic testing

    

Prnpcodon genotypesa

Genotypes for sample identificationb

GenBank

Acc. No.

MARC

animal no.

Breed

Sex

codons

136 154

171

codons

112 136 141 154

171 237

1c

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

AY907691

200423666

MARCIII Composite

ram

ARR, ARR

MALRRL, MALRRL

R

C

Y

T

R

W

G

T

G

R

G

K

W

G

R

G

Y

Y

R

C

DQ345759

200323140

MARCIII Composite

eve

ARR, ARQ

MALRRL, MALRQL

G

Y

Y

Y

A

T

T

Y

R

G

G

T

W

S

G

A

Y

C

R

Y

AY907682

199735002

Rambouillet

ram

ARR, AHQ

MALRRL, MALHQL

A

C

C

C

R

W

G

T

G

R

G

G

W

G

R

R

Y

C

G

T

AY907681

200450394

Texel cross

ram

ARR, ARH

MALRRL, MALRHL

R

T

Y

C

R

W

G

C

R

A

T

O

T

S

G

R

Y

Y

R

C

AY907688

200423500

MARCIII Composite

ram

ARR, VRQ

MALRRL, MVLRQL

A

Y

T

Y

A

T

G

C

R

R

K

G

W

S

A

R

Y

Y

G

T

EF189729

200665217

Navajo Churro cross

ewe

ARR, ARK

MALRRL, MALRKL

G

Y

Y

T

G

W

K

C

R

A

G

K

T

S

G

R

Y

C

A

Y

AY907689

200423510

MARCIII Composite

ram

ARQ, ARQ

MALRQL, MALRQL

R

C

C

Y

A

A

G

T

R

R

K

K

W

S

R

R

Y

T

A

Y

AY907687

200206039

Texel

ram

ARQ, AHQ

MALRQL, MALHQL

G

Y

Y

Y

R

A

G

Y

A

A

K

K

A

S

G

A

T

Y

A

C

AY907693

200450134

Texel cross

ram

ARQ, ARH

MALRQL, MALRHL

R

Y

Y

T

G

A

K

T

R

A

K

G

T

G

R

R

T

Y

G

C

AY907690

200423529

MARCIII Composite

ram

ARQ, VRQ

MALRQL, MVLRQL

R

T

Y

Y

G

T

K

C

R

R

T

G

A

C

A

R

T

Y

R

Y

EF189727

200665234

Navajo Churro cross

ram

ARQ, ARK

MALRQL, MALRKL

R

Y

Y

Y

G

W

G

Y

R

A

K

T

T

G

G

G

O

C

R

Y

AY909542

199935904

Rambouillet

ram

AHQ, AHQ

MALHQL, MALHQL

A

C

T

C

G

T

K

Y

G

R

K

K

A

C

G

R

Y

Y

A

Y

AY907692

200450114

Texel cross

ewe

AHQ, ARH

MALHQL, MALRHL

R

T

Y

C

R

W

G

Y

R

A

K

G

W

S

G

A

T

T

R

Y

AY907686

200177091

Rambouillet-Romanov

ewe

AHQ, VRQ

MALHQL, MVLRQL

R

Y

T

C

R

T

G

Y

R

G

T

G

W

S

G

R

Y

T

A

Y

EF189725

200665201

Navajo Churro cross

ram

AHQ, ARK

MALHQL, MALRKL

A

C

Y

Y

R

W

G

T

R

A

G

K

A

G

G

A

Y

C

R

Y

AY907683

200006024

Texel

ram

ARH, ARH

MALRHL, MALRHL

A

C

C

C

R

W

K

Y

G

A

T

K

A

G

G

A

O

C

R

C

AY907694

200450173

Texel cross

ewe

ARH, VRQ

MALRHL, MVLRQL

R

T

Y

Y

G

W

G

Y

G

A

T

G

W

S

G

R

Y

Y

R

C

EF189726

200665213

Navajo Churro cross

ram

ARH, ARK

MALRHL, MALRKL

A

Y

Y

T

G

T

G

C

A

A

K

T

A

G

R

G

O

C

A

Y

AY907685

200123331

MARCIII Composite

ewe

VRQ, VRQ

MVLRQL, MVLRQL

R

C

Y

Y

R

W

G

C

G

G

K

G

W

S

R

G

O

Y

R

C

EF189722

200665222

Navajo Churro cross

ewe

VRQ, ARK

MVLRQL, MALRKL

R

C

T

T

G

W

K

Y

R

R

K

K

A

G

G

G

Y

Y

R

Y

EF189728

200665931

Barbados Black Belly

ram

ARK, ARK

MALRKL, MALRKL

R

Y

C

C

R

T

G

Y

A

G

T

K

T

S

A

A

Y

Y

R

T

FJ404779

200117482

MARCIII Composite

ewe

ARQ, ARR

MALRQL, MALRRL

R

Y

C

Y

A

A

K

C

A

R

G

T

T

S

G

A

Y

C

A

T

FJ404777

200210034

MARCIII Composite

ewe

ARQ, ARQ

MALRQL, TALRQL

R

C

T

C

A

A

K

C

A

A

G

T

A

C

G

R

T

C

A

Y

FJ404778

200310125

MARCIII Composite

ewe

ARQ, ARQ

TALRQL, TALRQL

G

T

Y

Y

R

A

K

Y

R

A

K

T

A

G

A

R

C

C

A

Y

EF189723

200603590

Navajo Churro cross

ram

ARQ, ARQ

MALRQL, TALRQL

G

T

C

C

A

W

K

T

R

R

T

G

T

S

R

R

Y

Y

G

C

EF189724

200277004

MARCIII Composite

ewe

ARQ, ARQ

MALRQL, MAFRQL

R

Y

C

C

R

W

K

Y

G

A

G

T

A

C

R

G

Y

C

G

C

-d

-

-

-

ARQ, ARQ

MALRQL, MAFRQP

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

ARQ, ARQ

MAFRQL, MAFRQL

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

FJ404776

200865003

MARCIII Composite

ewe

ARQ, ARQ

MAFRQL, MAFRQP

R

T

C

T

R

W

T

C

A

A

G

G

W

S

R

R

Y

Y

R

T

GQ380576

200941201

MARCIII Composite

ram

ARQ, ARQ

MAFRQP, MAFRQP

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

  1. aThe predicted amino acid residues haplotypes inferred from those known to be segregating in these USMARC sheep populations.
  2. bAutosomal SNP markers were selected from those reported by Kijas et al. (PLoS ONE. 2009;4(3):e4668). The genotypes were obtained by Sanger sequencing. Homozygous genotypes are listed as a single letter corresponding to the nucleotide at those sites, whereas, IUB ambiguity codes denote heterozygous genotypes those site: Y = C,T; R = A,G; M = A,C; K = G,T; W = A,T; S = C,G. Genotypes listed as "O" indicate missing information. The SNP marker properties and details are available at this site: http://cgemm.louisville.edu/USDA/index.html. Marker 250506CS is ISGC EST 250506CS3900218700001.
  3. cMarker names: 1, CZ920950; 2, DU183112; 3, DU191809; 4, DU202116; 5, DU202534; 6, DU223430; 7, DU232778; 8, DU247686; 9, DU275655; 10, DU301854; 11, DU320019; 12, DU351298; 13, DU360304; 14, DU366451; 15, DU383209; 16, DU442796; 17, DU487949; 18, DU494996; 19, DU499024; 20, 250506CS3900218700001.
  4. dInformation expected to be available in fall of 2010.
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BMC Veterinary Research

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