Characterization of extracellular matrix macromolecules in primary cultures of equine keratinocytes

BMC Veterinary Research

Table 1 Optimization of culture conditions of lamellar keratinocytes

Growth Media	Calcium Concentration	Survival	Days to confluence (% confluence reached)	Ability to passage
KSFM + supp^a +2% FBS^b	0.074 mM	No	14 (few cells remain attached)	N/D
KSFM + supp +5% FBS	0.275 mM	Yes	18 (20%)	N/D
DMEM -- Ca²⁺+ supp + 2% FBS	0.074 mM	No	16 (few cells remain attached)	N/D
DMEM -- Ca²⁺+ supp + 5% FBS	0.185 mM	Yes	19.5 ± 2.12 (70%) (n = 2)	No
DMEM -- Ca²⁺+ supp + 5% FBS	0.6 mM^c	Yes	17.5 ± 0.71 (70%) (n = 2)	Yes

^a10 ng/ml EGF + 30 μg/ml BPE + 0.4 μg/ml hydrocortisone + 5 μg/ml insulin
^bFBS used had a calcium concentration of 3.7 mM (Thermo Scientific, Lot E08009)
^cMedia supplemented with additional calcium to 0.6 mM N/D = not determined

ISSN: 1746-6148