Western immunoblot of caudal medulla of selected pigs from each group.
1 Biotinylated molecular mass marker
No PK PK+ Animal ID
2 8 BSE group (positive) - PR463
3 9 BSE group (positive) - PR443
4 11 Pre-1996 group (negative) - PR469
5 12 Post-1996 group (negative) - PR476
6 13 BSE group (negative, clinical suspect) - PR442
7 14 Un-inoculated pig (negative) - PR459
10 NZ-brain (negative) - PR591
15 Bovine BSE positive control
16 Ovine scrapie positive control
VLA Hybrid technique (discriminatory WB) using core mAb, 6H4 (Figure A), and 'N' terminal mAbs, P4 (Figure B) and 12B2 (Figure C), to distinguish scrapie from BSE. Treatment with and without PK was used to visualise PrPres and PrPc respectively.