Figure 4
From: Transmissibility studies of vacuolar changes in the rostral colliculus of pigs
Western immunoblot of caudal medulla of selected pigs from each group.
Lanes
1Â Â Â Â Â Â Biotinylated molecular mass marker
No PKÂ Â Â PK+Â Â Â Animal ID
2Â Â Â Â Â Â Â Â Â 8Â Â Â Â Â Â BSE group (positive) - PR463
3Â Â Â Â Â Â Â Â Â 9Â Â Â Â Â Â BSE group (positive) - PR443
4Â Â Â Â Â Â Â Â Â 11Â Â Â Â Â Pre-1996 group (negative) - PR469
5Â Â Â Â Â Â Â Â Â 12Â Â Â Â Â Post-1996 group (negative) - PR476
6Â Â Â Â Â Â Â Â Â 13Â Â Â Â Â BSE group (negative, clinical suspect) - PR442
7Â Â Â Â Â Â Â Â Â 14Â Â Â Â Â Un-inoculated pig (negative) - PR459
          10     NZ-brain (negative) - PR591
          15     Bovine BSE positive control
          16     Ovine scrapie positive control
VLA Hybrid technique (discriminatory WB) using core mAb, 6H4 (Figure A), and 'N' terminal mAbs, P4 (Figure B) and 12B2 (Figure C), to distinguish scrapie from BSE. Treatment with and without PK was used to visualise PrPres and PrPc respectively.