Figure 2

Immunohistochemistry using the Swiss anti-rabies monoclonal antibody to detect Lyssavirus antigen in infected fox brain tissue. Histopathology legend: Panels A-C* were from an EBLV-1 infected animal inoculated via the IC route. Panels D-F were from an EBLV-2 infected animal inoculated via the IC route. Panels G-I were from an EBLC-1 infected animal inoculated via the IM route. Panels A, D and G were from the hippocampus and panels B, E and H were from the thalmic region. Panel C was from the cortex, panel F from the cerebellum and panel I was from the medulla. Arrows on panels F indicate antigen accumulation reminiscent of Negri bodies, and arrows on panels E and I indicate perivascular, inflammatory infiltrate is also evident in panels E, F, H and I. Arrows on panel G highlight rare positive cells in the hippocampus of an IM inoculated animal. *these sections have a higher 'coffee wash' background as the carcass was frozen prior to post-mortem.