Figure 1From: Expression levels of immune markers in Actinobacillus pleuropneumoniaeinfected pigs and their relation to breed and clinical symptomsKinetics of the activation of immune markers in vitro in response to A. pleuropneumoniae infection. H2O2 (A) and TNF-α (B) production were measured after in vitro stimulation of blood leukocytes with PMA (for A) or inactivated A. pleuropneumoniae (for B). (C) Basal expression of TNF-α by BALF leukocytes after 6 h cultivation in vitro. The expression of the specific markers was recorded as shown on the bottom of the graph for each breed at 7 days before infection (-7) as well as at 4 and 21 days after infection. The box represents the 50% between 25% and 75% quartiles. The line inside the box indicates the median. The top and bottom lines denote the 5 and 95 percentile, whereas the black crosses denote minimum and maximum values. The numbers on the top of the boxes indicate the number of samples examined. ND – not determined; * p < 0.05; ** p < 0.01; *** p < 0.001.Back to article page