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Table 2 Identification of proteins from equine plasma in reducing 1-D SDS-PAGE.

From: Biomarkers for ragwort poisoning in horses: identification of protein targets

Band No. Protein Identification Accession No. Mr (kDa) pI MOWSE Scorea
(p≤ 0.05)
No. of Peptides Matched % Coverageb Species
R1 Serum albumin CAA52194 70.5 5.95 437 (44) 48 30 Equus caballus
  Immunoglobulin gamma 7 heavy chain AAS18414 36.3 7.70 132 (44) 8 - Equus caballus
  Immunoglobulin gamma 4 heavy chain AAS18415 36.2 7.71 127 (44) 10 - Equus caballus
  Fibrinogen A α chain (fragment) AAC67561 49.6 6.45 49 (45) 3 7 Equus caballus
R2 Serum albumin CAA52194 70.6 5.95 639 (44) 39 31 Equus caballus
  Fibrinogen A α chain (fragment) AAC67561 49.6 6.45 230 (44) 14 19 Equus caballus
  Immunoglobulin G heavy chain (fragment) AAG01011 47.6 5.7 141 (44) 8 14 Equus caballus
  Immunoglobulin G light chain (fragment) AAG01010 24.5 6.4 141 (44) 4 9 Equus caballus
  Apolipoprotein A-1 P02648 30.2 5.2 94 (44) 6 7 Canis familiaris
R3 Transferrin AAA63684 80.3 6.83 685 (44) 47 32 Equus caballus
  Fibrinogen A α chain AAC67561 49.6 6.45 195 (44) 15 26 Equus caballus
  Serum albumin CAA52194 70.6 5.95 187 (44) 16 14 Equus caballus
  Immunoglobulin mu AAU09792 50.1 5.52 127 (44) 10 16 Equus caballus
R4 Serum albumin AAV28861 70.5 5.89 249 (45) 18 16 Equus caballus
  Fibrinogen A α chain AAC67561 49.6 6.45 50 (45) 3 5 Equus caballus
R5 Immunoglobulin gamma 7 heavy chain AAS18414 36.3 7.7 305 (44) 25   Equus caballus
  Immunoglobulin gamma 4 heavy chain constant region (fragment) CAC44763 37.0 8.04 280 (44) 24 32 Equus caballus
  Immunoglobulin gamma 5 heavy chain constant region (fragment) CAC86340 36.4 5.95 256 (44) 24 33 Equus caballus
  Immunoglobulin G heavy chain (fragment) AAG01011 47.6 5.7 256 (44) 22 21 Equus caballus
  Immunoglobulin gamma 4 heavy chain AAS18415 36.2 7.71 247 (44) 24 - Equus caballus
  Immunoglobulin gamma 1 heavy chain constant region (fragment) CAC44624 38.0 7.68 224 (44) 11 19 Equus caballus
  Immunoglobulin gamma 6 heavy chain constant region (fragment) CAC86341 36.5 8.03 212 (44) 13 24 Equus caballus
  Serum albumin CAA52194 70.6 5.95 92 (44) 6 4 Equus caballus
  Haptoglobin CAA25267 39.0 6.13 75 (45) 5 - Homo sapiens
R6 Immunoglobulin gamma 7 heavy chain AAS18414 36.3 7.7 305 (44) 25   Equus caballus
  Immunoglobulin gamma 4 heavy chain constant region (fragment) CAC44763 37.0 7.28 280 (44) 24 32 Equus caballus
  Immunoglobulin gamma 4 heavy chain AAS18415 36.2 7.71 247 (44) 24 -  
  Immunoglobulin gamma 1 heavy chain constant region (fragment) CAC44624 38.0 7.68 224 (44) 11 19 Equus caballus
  Immunoglobulin gamma 6 heavy chain constant region (fragment) CAC86341 36.5 8.03 212 (44) 13 24 Equus caballus
R7 Immunoglobulin gamma 4 heavy chain AAS18415 36.2 7.71 93 (44) 3 10 Equus caballus
  Immunoglobulin gamma 4 heavy chain constant region (fragment) CAC44763 37.0 7.28 93 (44) 3 10 Equus caballus
R8 Apolipoprotein A-1 P02648 30.1 5.2 608 (44) 36 27 Canis familiaris
  Lambda immunoglobulin (fragment) AAA50978 17.6 8.35 295 (44) 17 23 Equus caballus
  Lambda immunoglobulin (fragment) AAA50971 20.7 8.22 192 (44) 7 24 Equus caballus
  1. The plasma proteins were identified using LC-MS/MS. Proteins were identified on the basis of their MOWSE score using the MSDB database. Each band number refers to proteins identified in Figure 2B. a = MOWSE baseline significance score in brackets; b = for those with no percentage coverage value the database did not give this information.