Table 3 Primers for amplification of the microsatellites at the exact position of the candidate gene loci
From: A study of candidate genes for day blindness in the standard wire haired dachshund
Microsatellite | Forward primer (5'-3') | Reverse primer (5'-3') | Size of PCR-product (bp) | Nucleotide repeat | Location (bp) | Accession number |
|---|---|---|---|---|---|---|
CNGB3-FH2772 | CCCAAAGCACATCCTAATTC | GGAGTCTGCTTCTCCCTTTC | 168 | di | Chr29:35405042-35405217 | UniSTS 263646 |
CNGB3-C29.002 | TATTAAATCCCAGTCACCACCC | AGGTCCCAGACCGAGTCC | 208 | di | Chr29:36423257-36423471 | UniSTS 262830 |
CNGA3-GT19 | CCTCCCACTCTCCCCTCTAC | CCAGGGGAGCTTTTACAACA | 337 | di | Chr10:47307640-47307977 | BV729091 |
CNGA3-GT20 | GCAAGCAGTCCCGATTTTTA | TCAGCTTTGGTCATGCACTC | 304 | di | Chr10:47283009-47283313 | BV729076 |
GNAT2-GAAA16 | CCCATGCTTGGTTTAATGCT | GACTGTCCTGCCTTCCATGT | 350 | tetra | Chr6:45194834-45195184 | BV729077 |
GNAT2-CTT22 | CAGCTGGATTCTTCCCATGA | GCCCAAATTGCAAATCCTTA | 273 | tri | Chr6:45461591-45461864 | BV729078 |
ABCA4-FH2370 | CCTGAAAAATAGCTAGATGATGG | GTCTTTACCTGCCTATATAGCTGC | 380 | tetra | Chr6:58367514-58367919 | UniSTS 263571 |
ABCA4-TTTC16m | GGAATCAGTGGACTCATCCAA | GGGGATTGGACAGTGGTAGA | 238 | tetra | Chr6:58046419-58046656 | BV729079 |
RDH5-TAGA10 | GAAGATGACGATGATGATGAAGA | GCTGAAGGTAGACGCTGGAC | 286 | tetra | Chr10:3068645-3068931 | BV729080 |
RDH5-GAAA16 | CCCTGCTGTGAGGAGTCAGT | AGCCAGATGCAGGACTTGAT | 209 | tetra | Chr10:3002063-3002276 | BV729081 |
CORD8-FMO5-GAAA12 | CCACAAGTTGGGGTTTCAAG | CCCCTCCTCTCTCTCTTTCC | 204 | tetra | Chr9:60070458-60070662 | BV729082 |
CORD8-FMO5-TTTA10 | CCCAGGTGTCCCTATTTTCC | GCTCACTGGGGAGTCTGCT | 175 | tetra | Chr9:60654305-60654480 | BV729083 |
CORD9-RP1-ATm22 | CACTGGATGCACACAGATCC | GGTCCTTGAGAAGGAAGCTG | 296 | di | Chr29:9128657-9128953 | BV729084 |
CORD9-RP1-TTTC21m | TCCAGTAGGCGTCCTCTGAC | AGTCAATGGAGCCTGCAACT | 410 | tetra | Chr29:9011174-9011583 | BV729085 |
RPGRIP1-GAAA26 | TGTTACCTGTTCCAAAGTTGTTTT | AGTTACAGCCATGGGAATGC | 475 | tetra | Chr15:21287275-21287750 | BV729086 |
GUCY2D-TTCC15 | ACAATGGGCACATCTGTTGA | TTCTCCCTCTGCCTGTGTCT | 405 | tetra | Chr5:35459311-35459716 | BV729089 |
GUCY2D-GAAA17 | CCCCTCTTCTCCACTCTCCT | TCATATTCTTGCCCCAGTCC | 444 | tetra | Chr5:35514793-35515246 | BV729090 |
CRX-GAAA15m | TGGTCTCACATTCCCACTGA | AGAAGTGGCAGAGCACAGGT | 438 | tetra | Chr1:111096908-111097346 | BV729087 |
CRX-GT21 | ACCAGAACCAAAGGCAGATG | TCAGGGTTGGAGTTTTGAGC | 427 | di | Chr1:111076912-111077339 | BV729088 |