Figure 1From: Identification of differentially expressed genes in ileal Peyer's Patch of scrapie-infected sheep using RNA arbitrarily primed PCRLaser-assisted microdissection of follicles in the ileal PP. Nuclei of the tissue sections were stained with hematoxylin. After laser-assisted dissection of each area (a) the samples were collected (b) using an adhesive membrane and transferred to a cap of a 0.5 ml reaction tube and kept at -20°C until RNA extraction. Bar: 200 μm. (c) A section of RAP-PCR fingerprint showing follicles from control animals (lanes 1–2), and follicles from scrapie animals (lanes 3–4). Arrowheads show a band differentially amplified by RAP-PCR indicating an up-regulation in the follicles of the scrapie infected animals.Back to article page