Expression stability of commonly used reference genes in canine articular connective tissues

BMC Veterinary Research

Table 1 Canine cell lines (stimulated with LPS) reference genes ranked in order of increasing expression stability (Increases from top to bottom).

Number of remaining reference genes	Combined Cell lines	M Value	V Value	DH82 Cells	M Value	V Value	K6 Cells	M Value	V Value
9	ACTB	1.24	0.222	RPL13A	0.83	0.114	ACTB	1.03	0.263
8	HPRT	1.00	0.167	YWHAZ	0.78	0.115	RPL13A	0.64	0.113
7	YWHAZ	0.85	0.125	B2M	0.73	0.142	GAPDH	0.56	0.102
6	HMBS	0.79	0.137	GAPDH	0.62	0.169	HMBS	0.50	0.142
5	SDHA	0.73	0.156	TBP	0.43	0.126	B2M	0.32	0.073
4	RPL13A	0.65	0.175	ACTB	0.29	0.112	HPRT	0.29	0.109
3	TBP	0.54	0.207	HPRT	0.14	0.059	TBP	0.14	0.063
2 Most stably expressed	*B2M/GAPDH*	0.34		*HMBS/SDHA*	0.07		*SDHA/YWHAZ*	0.05

ISSN: 1746-6148