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Table 5 Results from trial 7–9; cattle faecal samples artificially contaminated with salmonella. Faeces originating from one cattle farm (Trial 7) and three cattle farms (Trial 8–9). For each trial different spiking levels of salmonella were constructed (Trial 7 S. Typimurium DT1, Trial 8 S. Dublin, Trial 9 S. Tennesse), each spiking level containing six 25 g samples of faeces

From: Comparison of culture, ELISA and PCR techniques for salmonella detection in faecal samples for cattle, pig and poultry

CFU salm/25 g NMKL MSRV MSRV PCR SELENIT CYSTINE VIDAS SLM SELECTA ELISA SELECTA CULTURE OPTIMA ELISA OPTIMA CULTURE BAX® SELECTA PCR SELECTA IMS PCR
TRIAL 7             
0 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6
15,000 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6
1,500 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6
150 6/6 6/6 6/6 6/6 0/6 6/6 6/6 0/6 3/6 6/6 6/6 6/6
15 3/6 6/6 6/6 6/6 0/6 6/6 6/6 0/6 0/6 6/6 6/6 6/6
TRIAL 8             
0 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6
400,000 2/6 N.P*** N.P*** 6/6 3/6 N.P*** N.P*** N.P*** N.P*** N.P*** N.P*** N.P***
82,000 0/6 6/6 6/6 6/6 0/6 6/6 6/6 0/6 0/6 N.P*** N.P*** N.P***
9,000 0/6 6/6 6/6 2/6 0/6 6/6 6/6 0/6 0/6 6/6 6/6 6/6
900 0/6 6/6 6/6 0/6 0/6 6/6 6/6 0/6 0/6 0/6 6/6 6/6
90 0/6 6/6 6/6 0/6 0/6 1/6 4/6 0/6 0/6 0/6 6/6 6/6
9 0/6 5/6 5/6 0/6 0/6 0/6 2/6 0/6 0/6 0/6 1/6 3/6
TRIAL 9             
0 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6
6,600 6/6 6/6 6/6 5/6 6/6 6/6 6/6 5/6 6/6 6/6 6/6 6/6
660 6/6 6/6 6/6 6/6 6/6 6/6 6/6 5/6 6/6 6/6 6/6 6/6
66 2/6 6/6 6/6 3/6 0/6 6/6 6/6 1/6 6/6 6/6 6/6 6/6
7 0/6 6/6 6/6 1/6 0/6 2/6 6/6 0/6 0/6 6/6 6/6 6/6
TP* 37 77 77 53 27 63 72 23 33 54 67 69
TN* 18 18 18 18 18 18 18 18 18 18 18 18
FP* 0 0 0 0 0 0 0 0 0 0 0 0
FN* 47 1 1 31 57 15 6 55 45 18 5 3
AC** 0.54 0.99 0.99 0.69 0.44 0.84 0.94 0.43 0.53 0.8 0.94 0.97
SE** 0.44 0.99 0.99 0.63 0.32 0.81 0.92 0.29 0.42 0.75 0.93 0.96
SP** 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
  1. *TP = True positives, TN = True negatives, FP = False positives, FN = False negatives
  2. **AC = Accuracy, SE = Sensitivity, SP = Specificity
  3. ***N.P = not performed