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Table 4 Results from trial 4–6; pig faecal samples artificially contaminated with salmonella. Faeces originating from three pig farms. For each trial different spiking levels of salmonella were constructed (Trial 4 S. Derby, Trial 5 S. Cubana, Trial 6 S. Typhimurium DT40), each spiking level containing six 25 g samples of faeces.

From: Comparison of culture, ELISA and PCR techniques for salmonella detection in faecal samples for cattle, pig and poultry

CFU salm/25 g NMKL MSRV MSRV PCR VIDAS SLM SELECTA ELISA SELECTA CULTURE OPTIMA ELISA OPTIMA CULTURE BAX® SELECTA PCR SELECTA IMS PCR
TRIAL 4            
0 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6
100,000 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6
10,000 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6
1000 3/6 6/6 6/6 6/6 6/6 6/6 4/6 6/6 6/6 6/6 6/6
100 0/6 6/6 6/6 3/6 6/6 6/6 0/6 4/6 6/6 6/6 6/6
10 0/6 6/6 6/6 5/6 5/6 4/6 0/6 0/6 6/6 6/6 6/6
TRIAL 5            
0 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6
12,000 6/6 6/6 6/6 6/6 6/6 6/6 3/6 6/6 6/6 6/6 6/6
1200 6/6 6/6 6/6 6/6 6/6 6/6 1/6 2/6 6/6 6/6 6/6
120 5/6 6/6 6/6 5/6 4/6 6/6 0/6 0/6 6/6 6/6 6/6
12 1/6 6/6 6/6 0/6 1/6 6/6 0/6 0/6 4/6 6/6 5/6
TRIAL 6            
0 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6
430,000 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6
38,000 0/6 6/6 6/6 5/6 2/6 5/6 0/6 0/6 5/6 6/6 6/6
3,800 0/6 6/6 6/6 0/6 0/6 1/6 0/6 0/6 6/6 2/6 3/6
380 0/6 6/6 6/6 0/6 0/6 0/6 0/6 0/6 6/6 0/6 1/6
38 0/6 2/6 2/6 0/6 0/6 0/6 0/6 0/6 5/6 0/6 0/6
TP* 38 80 80 54 54 54 26 36 80 68 69
TN* 18 18 18 18 18 18 18 18 18 18 18
FP* 0 0 0 0 0 0 0 0 0 0 0
FN* 46 4 4 30 30 30 58 48 4 15 15
AC** 0.54 0.96 0.96 0.71 0.71 0.71 0.43 0.53 0.96 0.82 0.85
SE** 0.45 0.95 0.95 0.64 0.64 0.64 0.31 0.42 0.95 0.77 0.82
SP** 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
  1. *TP = True positives, TN = True negatives, FP = False positives, FN = False negatives
  2. **AC = Accuracy, SE = Sensitivity, SP = Specificity