Table 2 PCR primers and parameters
From: Rapid detection of Mycobacterium bovis DNA in cattle lymph nodes with visible lesions using PCR
PCR | Primer | Sequence | [Mg] mM | Anneal temp. | Amplicon size (bp) | T melt Celsius |
|---|---|---|---|---|---|---|
Bos taurus | cytb F | 5'-CCATCGAACATTTCATCATGATGGAA-3' | 2.0 | 64 | 359 | 95 |
| Â | cytb R | 5'-GCTCCTCAGAATGATATTTGTCCTCA-3' | Â | Â | Â | Â |
IS1081 | F2 | 5'-CTGCTCTCGACGTTCATCGCCG-3' | Â | Â | 135 | 94 |
| Â | R2 | 5'-GGCACGGGTGTCGAAATCACG-3' | 2.0 | 58 | 113 | Â |
| Â | R3 | 5-TGGCGGTAGCCGTTGCGC-3 | Â | Â | Â | Â |
RD4 flanking | F1 | 5'-AATGGTTTGGTCATGACGCCTTC-3' | Â | 58 | 176 | 92 |
| Â | F2 | 5-TGTGAATTCATACAAGCCGTAGTC-3 | 2.0 | Â | 142 | Â |
| Â | R1 | 5'-CCCGTAGCGTTACTGAGAAATTGC-3' | Â | Â | Â | Â |