A proteomics study of the response of North Ronaldsay sheep to copper challenge

BMC Veterinary Research

Table 2 Identification of copper-responsive proteins in Cambridge sheep. 2-D gels for each animal were run in triplicate and placed in analysis sets. Statistical analysis sets were created from two copper-challenged Cambridge (n = 6 gels) and North Ronaldsay (n = 6 gels) animals and statistically significant changes in protein expression were identified by Student's t-test at p ≤ 0.05.

Protein Identification	Accession	Species	Mwt (kDa)	Mowse score	Blast score	E value	Seq coverage	Fold change (%)
2a. Epoxide hydrolase	[PIR:A47504]	Mouse	62.6	-	29	9.3	(LG)ASLNTPFMQAVP	3
	[EMBL:AAS68016]	Pig		-	25	238	SSDETFLTVSR
		Several			25	142	(YQ)LPALAQ(FQ)R
4. Heat shock protein Hsp 27	[Swiss-Prot:JC4244]	Dog	22.9	86			29%	N/A
5. Cytosolic NADP⁺-dependent isocitrate dehydrogenase	[trEMBL:Q6XUZ5]	Sheep	47.2	272			61%	N/A
6. Methionine adenosyl transferase	[Swiss-Prot:S06114]	Rat	44.2	129			27%	2.6

2-D gels for each animal were run in triplicate and placed in analysis sets. Statistical analysis sets were created from two copper-challenged Cambridge and North Ronaldsay animals and statistically significant changes in protein expression were identified by Student's t-test at p ≤ 0.05.

ISSN: 1746-6148