A proteomics study of the response of North Ronaldsay sheep to copper challenge

BMC Veterinary Research

Table 1 Identification of copper-responsive proteins in North Ronaldsay sheep. 2-D gels for each animal were run in triplicate and placed in analysis sets. Statistical analysis sets were created from two copper-challenged Cambridge (n = 6 gels) and North Ronaldsay (n = 6 gels) animals and statistically significant changes in protein expression were identified by Student's t-test at p ≤ 0.05.

Protein Identification	Accession	Species	Mwt (kDa)	Mowse score	Blast score	E value	Seq coverage	Fold change
1. Cathepsin D	[Swiss-Prot:Q9MZS8]	Sheep	39.8		33	0.78	VSSLPQVTLK	4
2b Epoxide hydrolase	[PIR:A47504]	Mouse	62.6		35	0.1	(AV)ASLNTPFM	N/A
	[EMBL:CAA46211.1]	Rat			41	0.002	YQLPALAQAGFR
3. Ferritin light chain	[Uniref:O46415]	Cow	19.9	112			31%	4
7. Peroxiredoxin 3 (SP-22)	[PIR:JC2258]	Cow	21.7	74			30%	10.0
		Rat	28.3		38	0.031	HLSVNDLPVGR
8. Plasma retinol binding protein	[PIR:S13186]	Cow	21.4	130			30%	2.8

2-D gels for each animal were run in triplicate and placed in analysis sets. Statistical analysis sets were created from two copper-challenged Cambridge and North Ronaldsay animals and statistically significant changes in protein expression were identified by Student's t-test at p ≤ 0.05.

ISSN: 1746-6148