Table 1 Primers used for mapping and tissue-specific expression of pig AKR1C genes.
Marker | GenBank Accession1 | TIGR Contig | Forward primer (5'→3') | Reverse primer (5'→3') | Application | Size (bp) |
|---|---|---|---|---|---|---|
AKR1CL2 | DQ494488 | TC204964 | gcgtctattacctccttgcatc | acctcgttctcattgtggtaca | RH mapping | 441 |
AKR1C1 | BV677932 | TC223177 | ggagtgttttctctccatggtc | cctatttcaatgatccacacga | RH mapping | 322 |
AKR1C2 | BV680531 | None | atcccaaagccagtgtctg | ttacatctgatctgccaaggtg | RH mapping | 348 |
AKR1C3 | DQ494490 | TC200330 | taagggcagacacagcttgata | tggaatacaaaactgaggcaga | RH mapping | 279 |
AKR1C4 | BV680541 | TC200328 | atcatcacctagggtcaacaattc | gagctcccgctcagtactcttcag | RH mapping | 345 |
SB89 microsatellite | DQ487182 | None | ttccacaccctctccttctcta | tgaaattcagttggaaagttttga | RH/linkage map | 150 |
SB90 microsatellite | DQ487183 | None | gggaattccatatgccatgagt | aagctgcagaaagagggaagta | RH/linkage map | 117 |
SB91 microsatellite | DQ487184 | None | aaattgaggaggagggaacagt | gtcaacaagatatggcctgta | RH/linkage map | 165 |
AKR1CL2 | DQ474064 | TC204964 | acccataccttacgcagaagaa | aaattctcaagaatccgctttg | RT-PCR | 240 |
AKR1C1 | DQ474065 | TC223177 | cgatggtcacttcattcctgta | agtgcttttccgttttcatctg | RT-PCR | 380 |
AKR1C2 | DQ474066 | None | tccttcgaccagagttggtcca | catttgacttgcagaaatccag | RT-PCR | 364 |
AKR1C3 | DQ474067 | TC200330 | ccattcagatgaaaattgcaga | ctcgttcttgcacttctccat | RT-PCR | 280 |
AKR1C4 | DQ474068 | TC200328 | aagacagagtgagcccagccg | cacctcctcctcgttttcatag | RT-PCR | 215 |