Porcine congenital splayleg is characterised by muscle fibre atrophy associated with relative rise in MAFbx and fall in P311expression

Table 1 Sequences of primers and TaqMan probes

Gene	Primer	Sequence 5' → 3'
Porcine MAFbx	S	AAGCGCTTCCTGGATGAGAA
	A	GGCCGCAACATCATAGTTCA
	P	AGCGACCTCAGCAGTTACTGCAACAAGG
Porcine P311	S	GAG GGA AGG CCT AAG GG
	A	CGG TCT CGC CAT CCT TCT T
	P	ACT TCC CAT CCC AAA GGA AGT GAA CCG
Murine P311	S	CAGCCAAGAACCGTTTGCAT
	A	CACTTCCTTAGGCACGGGAA
	P	TTCCCTTAATAAGACCTCCCTCCATTTCCTTG
Murine MYHslow	S	GCC TGG GCT TAC CTC TCT ATC AC
	A	CTT CTC AGA CTT CCG CAG GAA
	P	CGT TTG AGA ATC CAA GGC TCA
Murine MYH2a	S	CAG CTG CAC CTT CTC GTT TG
	A	CCC GAA AAC GGC CAT CT
	P	TGA GTT CAG CAG TCA TGA G
Murine MYH2x	S	GGA CCC ACG GTC GAA GTT G
	A	GGC TGC GGG CTA TTG GTT
	P	CTA AAG GCA GGC TCT CTC ACT GGG CTG
Murine MYH2b	S	CAA TCA GGA ACC TTC GGA ACA C
	A	GTC CTG GCC TCT GAG AGC AT
	P	TGC TGA AGG ACA CAC AGC TGC ACC T
Murine Myf5	S	CAG CCC CAC CTC CAA CTG
	A	GCA GCA CAT GCA TTT GAT ACA TC
	P	TGT CTG GTC CCG AAA GAA CAG CAG CTT
Murine MYHembryonic	S	TCC GAC AAC GCC TAC CAG TT
	A	CCC GGA TTC TCC GGT GAT
	P	ATG CTG ACT GAT CGT GAG AAC CAG TCT ATC CT
Murine MYHperinatal	S	GGA GGC CAG GGT ACG TGA A
	A	GAG CAC ATT CTT GCG GTC TTC
	P	AGG AAC TTA CCT ACC AGA CTG
Murine α-actin	S	GAG CGT GGC TAT TCC TTC GT
	A	CAC ATA GCA CAG CTT CTC TTT GAT
	P	CGC GCA CAA TCT CAC GTT CAG CTG
Murine β-actin	S	CGT GAA AAG ATG ACC CAG ATC A
	A	CAC AGC CTG GAT GGC TAC GT
	P	TTG AGA CCT TCA ACA CCC CAG CCA TG

S, sense; A, antisense; P, TaqMan probe. All probes were 5' labelled with FAM, and 3' labelled with TAMRA.

ISSN: 1746-6148