Figure 3
From: Rapid and discriminatory diagnosis of scrapie and BSE in retro-pharyngeal lymph nodes of sheep
Typical PrPresprofiles in scrapie and BSE infected retropharyngeal lymph nodes using different antibodies. Type of specimen applied: lanes 1 and 7 molecular weight markers in kDa; lane 2 brain stem homogenate from ARQ/VRQ sheep with scrapie; lane 3, RLN from VRQ/VRQ scrapie sheep at age 26 m; lane 4, RLN from BSE-infected ARQ/ARQ sheep in preclinical stage of disease; lane 5, RLN from ARQ/ARQ sheep with subclinical (brain positive, no clinical signs) stage of scrapie; lane 6, RLN from BSE-infected ARQ/ARQ sheep in clinical stage of disease. Amounts of tissue applied: lane 2, 200 μg TE, lanes 3–6, 10 mg TE. Antibody concentrations used: L42 and 12B2, 0.2 μg/ml; 9A2, 2 μg/ml.