Figure 4From: Sambucus nigra extracts inhibit infectious bronchitis virus at an early point during replicationTreating IBV with S. nigra extracts prior to infection is necessary for full virus inhibition and works synergistically with treating cells after infection. Cells and virus were treated with 4.0 x 10-3Â g/ml of S. nigra extract as indicated below. Infection was done at an MOI of 0.1. Quantitation of virus titers at 24Â h p.i. was done by plaquing in duplicate using neutral red staining. Independent infections with IBV were performed three times, with two replicates per assay.Back to article page