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Table 2 Reproducibility of PPRV N gene based real time RT-PCR assay

From: A SYBR Green I based real time RT-PCR assay for specific detection and quantitation of Peste des petits ruminants virus

a. Intra-assay variation
DNA copies/μl   Ct values   Mean Ct S.D
5.11 × 107 10.71 10.77 10.89 10.79 0.09
5.11 × 106 14.52 14.81 14.75 14.69 0.15
5.11 × 105 18.70 18.91 18.22 18.61 0.35
5.11 × 104 21.77 21.75 21.58 21.70 0.10
5.11 × 103 25.56 24.45 24.74 24.92 0.58
5.11 × 102 27.07 27.27 26.89 27.08 0.19
5.11 × 101 30.57 30.74 30.57 30.63 0.10
5.11 × 10° 32.52 33.26 32.59 32.79 0.41
b. Inter-assay variation
DNA copies/μl   Ct values   Mean Ct S.D.
Assay 1 Assay 2 Assay 3
5.11 × 107 10.75 10.79 11.24 10.93 0.27
5.11 × 106 14.84 14.70 16.17 15.24 0.81
5.11 × 105 18.92 18.61 20.33 19.29 0.92
5.11 × 104 22.07 21.7 23.14 22.30 0.75
5.11 × 103 24.67 24.91 24.57 24.72 0.17
5.11 × 102 27.42 27.08 27.61 27.37 0.27
5.11 × 101 31.13 30.63 32.83 31.53 1.15
5.11 × 10° 33.12 32.79 33.74 33.22 0.48