a. Intra-assay variation | |||||
---|---|---|---|---|---|
DNA copies/μl | Ct values | Mean Ct | S.D | ||
5.11 × 107 | 10.71 | 10.77 | 10.89 | 10.79 | 0.09 |
5.11 × 106 | 14.52 | 14.81 | 14.75 | 14.69 | 0.15 |
5.11 × 105 | 18.70 | 18.91 | 18.22 | 18.61 | 0.35 |
5.11 × 104 | 21.77 | 21.75 | 21.58 | 21.70 | 0.10 |
5.11 × 103 | 25.56 | 24.45 | 24.74 | 24.92 | 0.58 |
5.11 × 102 | 27.07 | 27.27 | 26.89 | 27.08 | 0.19 |
5.11 × 101 | 30.57 | 30.74 | 30.57 | 30.63 | 0.10 |
5.11 × 10° | 32.52 | 33.26 | 32.59 | 32.79 | 0.41 |
b. Inter-assay variation | |||||
DNA copies/μl | Ct values | Mean Ct | S.D. | ||
Assay 1 | Assay 2 | Assay 3 | |||
5.11 × 107 | 10.75 | 10.79 | 11.24 | 10.93 | 0.27 |
5.11 × 106 | 14.84 | 14.70 | 16.17 | 15.24 | 0.81 |
5.11 × 105 | 18.92 | 18.61 | 20.33 | 19.29 | 0.92 |
5.11 × 104 | 22.07 | 21.7 | 23.14 | 22.30 | 0.75 |
5.11 × 103 | 24.67 | 24.91 | 24.57 | 24.72 | 0.17 |
5.11 × 102 | 27.42 | 27.08 | 27.61 | 27.37 | 0.27 |
5.11 × 101 | 31.13 | 30.63 | 32.83 | 31.53 | 1.15 |
5.11 × 10° | 33.12 | 32.79 | 33.74 | 33.22 | 0.48 |