Table 1 Average number of immunopositive neurons in each brain area per treatment group and results of statistical analyses
Brain area | Neuron type | CL NO | CL HEN | LML NO | LML HEN | Main effect: Hen rearing | Main effect: Genetic line | Interaction: Hen-rearing by Genetic line | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Number of immunopositive neurons | F | p | F | p | F | p | |||
PaPC | AVT* | 108.0 ± 12.7 | 148.6 ± 29.5 | 140.1 ± 16.7 | 123.7 ± 38.4 | 0.00 | 1.00 | 0.05 | 0.82 | 2.39 | 0.14 |
| Â | TH | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
PaMC | AVT | 65.0 ± 12.5 | 104.9 ± 26.9 | 68.1 ± 13.7 | 61.3 ± 10.1 | 0.02 | 0.89 | 0.76 | 0.39 | 2.02 | 0.17 |
| Â | TH | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
whole PVN | TH | 39.3 ± 8.1 | 49.1 ± 3.1 | 44.9 ± 3.4 | 39.8 ± 7.7 | 0.00 | 0.95 | 0.50 | 0.49 | 1.04 | 0.32 |
MPO | AVT | 30.4 ± 8.2 | 35.9 ± 10.9 | 24.0 ± 7.8 | 29.4 ± 7.9 | 0.18 | 0.68 | 0.56 | 0.46 | 0.18 | 0.68 |
| Â | TH | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
MPA | AVT | 6.3 ± 2.8 | 28.4 ± 12.7 | 5.9 ± 3.8 | 37.7 ± 9.9 | 6.70 | 0.02 | 0.45 | 0.51 | 1.07 | 0.31 |
| Â | TH | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
AMPO | AVT | 18.4 ± 5.3 | 32.4 ± 5.4 | 31.7 ± 6.8 | 31.4 ± 6.4 | 1.63 | 0.21 | 1.08 | 0.31 | 1.88 | 0.18 |
| Â | TH | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
LPO | AVT | 60.6 ± 10.1 | 72.1 ± 10.5 | 56.1 ± 13.0 | 97.7 ± 19.5 | 4.25 | 0.05 | 0.52 | 0.48 | 0.59 | 0.45 |
| Â | TH | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
whole POA | TH | 26.7 ± 16.0 | 5.9 ± 5.9 | 36.0 ± 13.9 | 15.3 ± 11.5 | 2.58 | 0.12 | 1.04 | 0.32 | 0.00 | 0.97 |
SON | AVT | 177.1 ± 52.8 | 172.9 ± 18.6 | 173.9 ± 40.7 | 168.1 ± 41.8 | 0.00 | 0.97 | 0.21 | 0.65 | 0.17 | 0.68 |
| Â | TH | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
AH | AVT | 16.1 ± 6.5 | 37.7 ± 9.9 | 15.3 ± 5.0 | 17.1 ± 5.2 | 2.15 | 0.16 | 1.44 | 0.24 | 1.88 | 0.18 |
|  | TH | 16.7 ± 5.2 | 16.1 ± 2.6 | 13.9 ± 1.1 | 11.3 ± 4.3 | 0.08 | 0.78 | 1.35 | 0.26 | 1.26 | 0.27 |
LH | TH | 20.9 ± 3.9 | 30.1 ± 6.8 | 30.1 ± 5.6 | 30.5 ± 6.6 | 0.30 | 0.59 | 0.60 | 0.45 | 0.29 | 0.60 |
VMH | AVT | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
|  | TH | 2.1 ± 0.6 | 3.9 ± 1.0 | 5.9 ± 2.3 | 2.5 ± 0.6 | 0.02 | 0.88 | 0.15 | 0.71 | 1.27 | 0.27 |
PH | AVT | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
|  | TH | 25.6 ± 5.2 | 22.9 ± 7.0 | 28.6 ± 5.3 | 23.0 ± 4.8 | 0.44 | 0.51 | 0.00 | 0.95 | 0.00 | 0.94 |
DM | AVT | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
|  | TH | 2.0 ± 1.2 | 3.6 ± 1.7 | 0.7 ± 0.5 | 3.7 ± 1.5 | 5.65 | 0.03 | 0.28 | 0.60 | 0.44 | 0.52 |
HSOD | TH | 6.7 ± 3.6 | 8.4 ± 1.9 | 6.4 ± 1.2 | 10.8 ± 3.6 | 1.48 | 0.24 | 0.30 | 0.59 | 0.23 | 0.64 |
Pe | AVT | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
|  | TH | 15.0 ± 3.2 | 13.9 ± 3.4 | 26.4 ± 3.2 | 24.5 ± 6.9 | 0.41 | 0.53 | 7.09 | 0.01 | 0.14 | 0.71 |
ML/MM | AVT | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
|  | TH | 1.3 ± 1.3 | 3.7 ± 3.2 | 4.4 ± 2.8 | 5.3 ± 4.8 | 0.31 | 0.58 | 0.41 | 0.53 | 0.63 | 0.44 |
RM | AVT | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
|  | TH | 12.4 ± 3.0 | 24.4 ± 6.7 | 24.7 ± 4.8 | 19.5 ± 2.1 | 0.78 | 0.39 | 1.71 | 0.20 | 2.26 | 0.15 |
maVTA | AVT | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
|  | TH | 0.0 ± 0.0 | 0.0 ± 0.0 | 27.7 ± 18.2 | 10.2 ± 10.2 | 0.32 | 0.58 | 3.66 | 0.07 | 0.35 | 0.56 |