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Table 1 Detection of anti-HEV antibody in swine sera by ELISA-ORF2, ELISA-kit, and Western blotting assays

From: Detection of serum antibodies to hepatitis E virus in domestic pigs in Italy using a recombinant swine HEV capsid protein

ELISA -ORF2 (%)* ELISA-kit (%) Western blotting (%) S/CO ELISA-ORF2 p S/CO ELISA-kit p Observed frequency Estimated frequency Probability of infection**
+ + + 5.40 <0.01 5.15 <0.05 75 71 1
+ + - 3.79   4.61   23 23 0.89
+ - + 4.69 <0.05 1.51 <0.05 4 5 0.98
+ - - 2.30   1.31   2 3 0.56
- + + 0.99 <0.05 2.10 <0.05 1 3 0.93
- + - 0.73   3.69   3 3 0.33
- - + 0.81 <0.05 1.80 <0.05 1 0 0.61
- - - 0.63   1.75   2 2 0.05
104 (93.7) 102 (91.9) 81 (73.0)      111   
  1. *positive/total tested.
  2. **estimated by the model.