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Figure 2 | BMC Veterinary Research

Figure 2

From: Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms

Figure 2

Cytograms of the ADVIA 120 for cat samples. See Figure 1 for remainder of key. (A) Cytograms without any abnormalities. (B) Suspicion of left shift: In the baso cytogram (B2), an indistinct separation between the mononuclear and polymorphonuclear cell populations is noted. In the peroxidase cytogram (B1), few cells are scattered upwards from the neutrophil population, extending the scale of the y-axis and accumulating on the upper boundary of the cytogram, which indicates large and possibly toxic neutrophils. (C) No clear separation of cell populations: in the peroxidase cytogram (C1), numerous platelet aggregates are noted spreading into the neutrophil and lymphocyte gate. In the baso cytogram (C2), a “shortened” population of polymorphonuclear cells indicative of a left shift is present. (D) No clear separation of cell populations and suspicion of left shift: in the peroxidase cytogram (D1), the neutrophil population is shifted towards the upper left of the neutrophil gate, extending into the LUC gate, which is indicative of myeloperoxidase deficiency of the neutrophils. In the baso cytogram (D2), polymorphonuclear and mononuclear cells are not clearly separated. (E) Suspicion of atypical lymphocytes: in the peroxidase cytogram (E1), an increased number of cells are present in the LUC gate. In the baso cytogram (E2), few cells are scattered from the mononuclear area upwards into the lyse-resistant area. (F) Suspicion of blasts: in the peroxidase cytogram (F1), a large cell population extending from the lymphocyte gate into the LUC gate indicating blast cells are present; in the baso cytogram (F2), many cells are scattered from the mononuclear area into the lyse-resistant area.

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