Figure 6From: Comparative study of the effects of fetal bovine serum versus horse serum on growth and differentiation of primary equine bronchial fibroblastsSera effects on β-actin and α-SMA expression. Western blots were performed on whole cell lysate from EBF cultured for 4 days in DMEM containing 10% FBS or HS. Densitometry of β-actin and α-SMA was calculated by SynGene. (A) EBF cultured in HS show a higher α-SMA expression than EBF cultured in FBS (n = 5). Serum batch of (B) FBS and (C) HS (n = 2) did not affect α-SMA expression. F1 and F2 represent FBS batches, and H1 and H2 the HS batches. Data are presented as means ± SEM. (*p < 0.01).Back to article page