Antigenicity characterization of the purified recombinant Cap
protein by Western blot analysis using PiCV-positive sera. Lane M, pre-stained protein marker; lane 1 to lane 5, PiCV-positive sera were used in Western blotting and the recognized position of rCapopt protein is indicated by a solid triangle. The pigeons that had PiCV-positive sera were identified as virus-positive animals using a PCR detection methods as described in a previous study . Lane 6, Pigeon normal serum was used as control.