The culturable intestinal microbiota of triploid and diploid juvenile Atlantic salmon (Salmo salar) - a comparison of composition and drug resistance

Background With the increased use of ploidy manipulation in aquaculture and fisheries management this investigation aimed to determine whether triploidy influences culturable intestinal microbiota composition and bacterial drug resistance in Atlantic salmon (Salmo salar). The results could provide answers to some of the physiological differences observed between triploid and diploid fish, especially in terms of fish health. Results No ploidy effect was observed in the bacterial species isolated, however, triploids were found to contain a significant increase in total gut microbiota levels, with increases in Pseudomonas spp., Pectobacterium carotovorum, Psychrobacter spp., Bacillus spp., and Vibrio spp., (12, 42, 9, 10, and 11% more bacteria in triploids than diploids, respectively), whereas a decrease in Carnobacterium spp., within triploids compared to diploids was close to significant (8% more bacteria in diploids). With the exception of gentamicin, where no bacterial resistance was observed, bacterial isolates originating from triploid hosts displayed increased resistance to antibacterials, three of which were significant (tetracycline, trimethoprim, and sulphonamide). Conclusion Results indicate that triploidy influences both the community and drug resistance of culturable intestinal microbiota in juvenile salmon. These results demonstrate differences that are likely to contribute to the health of triploid fish and have important ramifications on the use of antibacterial drugs within aquaculture.


Background
Artificial triploid fish, that possess three complete sets of chromosomes compared to the more natural state of diploidy that possess two complete chromosome sets, are sterile due to reduced gonadal development and non-functional gametes [1]. Consequently, triploids are appealing to the aquaculture industry as a method for eliminating genetic interactions between wild and cultured stocks and reducing the financial costs associated with early maturation [2,3]. Today, triploids (mainly salmonids) are commercially produced in several countries around the globe including France and Australia [1,4].
Previously, is has been commented that the physiology of triploids is sufficiently different to diploids that they should be treated as a separate species [5]. Of most interest, triploids are composed of cells that are typically 50% larger than diploids due to the accommodation of the extra genetic material within the cell nucleus [6]. However, no size increase of the fish body is achieved through triploidy as the majority of tissues are composed of fewer cells, resulting in similar organ and whole body sizes between the ploidies [6][7][8]. The reduction in cell number may reduce oxygen delivery [9] and there is also evidence to suggest triploids have a lower optimum metabolic temperature compared to diploids [10], altered nutritional requirements [11], and differences in ontogenetic growth [1]. It is also widely reported that triploids are more susceptible to stress and disease [12][13][14][15], although this has yet to be confirmed in controlled experiments [16][17][18][19]. In addition, in contrast to male triploids, triploid females typically show no morphological or physiological signs of sexual maturation [8].
Evidence from both mammalian and fish studies suggests the gut microbiota is both influenced by, and has an influence on, host physiology [20][21][22][23]. For instance, whilst the gastrointestinal (GI) tract provides an ecological niche for bacterial growth [20], the microbiota present within the GI tract is dependent on host species, strain, ontogenetic growth, stress levels, and gut structure, whereas nutrient processing and absorption, development of the mucosal immune system, angiogenesis, and epithelial renewal are influenced by the gut microbiota [20][21][22][23][24][25]. Therefore, the gut microbiota plays a significant role in an individual's physiological status. As several of the above listed host factors are either known or suspected to be affected by triploidy (ontogenetic growth, stress response, immunological functioning, and gut structure/cell size and number), it is likely that gut microbiota is also affected by triploidy. Significantly, a changed microbiota may ease the growth and survival of potential fish pathogenic bacteria and also result in a different maturation of the mucosal immunity in young fish.
Previous studies have focused on characterizing the microbiota of different fish species and the biological significance of those bacteria present. For instance, the GI microbiota of several commercially important species has been studied during various life stages, such as in the salmonids [20]. Subsequently, bacteria have been classified as either allochthonous (transient) or autochthonous (permanent), with the allochthonous bacteria supplying the stock from which the autochthonous bacteria may become established. In addition, some bacteria have been identified as pathogens [i.e. Vibrio; [26]], involved in enteritis [27], or inhibit the growth of other bacteria [Carnobacterium spp.; [28,29]]. These findings have led to attempts to manipulate the gut microflora of fish through pre-and probiotics to improve fish health and growth [30].
A current area of concern within the field of environmental microbiology is the increase in the occurrence of antibiotic resistance among bacteria related to animal production. In this context it is considered that drug resistant bacteria in the environment may potentially transfer their resistance features to not only animal but also human pathogens [31]. This assumption has led to intensified efforts to monitor bacterial resistance, and is extensively done within bacteria of production animals and their environments [ [32,33], monitoring schemes, Norway NORM/NORM-VET, Denmark DANMAP, Sweden SVARM]. Significantly, bacteria are capable of transferring antibiotic resistance both within and between species through processes such as conjugation of transferable R-plasmids [34,35]. Traditionally, the use of antibiotics was assumed the main selection factor for increasing antibiotic resistance in aquaculture and the surrounding environment [36,37]; however, recent evidence would suggest that feed and just the presence of an aquaculture facility may also influence the antibiotic resistance levels of bacteria [38,39].
Differences in physiology related to ploidy may influence the gut microbial community and therefore, through a changed microbiota, secondarily affect the growth and health of triploid fish; two key elements in successful aquaculture, however no information is currently available. The objective of this experiment was to compare the culturable intestinal microbiota of cultured triploid and diploid juvenile Atlantic salmon (Salmo salar) for differences in the microbial community and the antibacterial resistance profiles of the bacteria based on primary traditional bacterial culturing.

Methods
The present experiment was approved by the Norwegian Animal Research Authority and performed according to prevailing animal welfare regulations.

Fish stock and rearing conditions
Fish eggs were provided by Aquagen (Trondheim, Norway) and reared at Matre Research Station, Norway. On Nov 03 2009, eggs (12,000-22,500/female, n = 209,700) from twelve female Atlantic salmon were fertilized by three different males, each male crossed with four different females (male 1 crossed with females 1-4, male 2 with females 5-8 etc), leaving four half-sibling families per male and three groups of independent families. Hydrostatic pressure, which interferes with the release of the second polar body, was used to create triploids [1,40]. Thirty-seven minutes and 30 seconds after fertilization at 8°C, half of the eggs from each female were subjected to a hydrostatic pressure of 655 bar for 6 min and 15 s (TRC-APV, Aqua Pressure Vessel, TRC Hydraulics inc., Dieppe, Canada), giving a total of twenty four groups (12/ploidy). Thereafter, each group was incubated in single incubation trays [Sterner 2003, Sterner Fish Tech AS] in an isolated (UV treatment) flow-through system (water was buffered with seawater to 0.7 ppt salinity; oxygen saturation 95%; pH 6.9) under darkness. The eggs were mechanically agitated to allow dead eggs to be sorted from live eggs at the eye-egg stage on December 21. Eggs from one of the females showed very high mortality at this stage, both as diploid and triploid, and were excluded from the study. After removing the dead eggs (mean 6.7% per female, min 0.9%, max 21%) in each of the remaining twenty two trays, all the family groups within each ploidy were mixed (1 L per female) in a bucket and re-distributed back into six trays (1 L eggs/tray; n = 3 trays/ploidy) leaving three trays/ploidy that each contained a mixture of all three independent families. Hatching started on February 05 2010, and was completed by February 18. On April 26 2010, the yolk sack larvae of each incubation tray were put into single, square, grey, covered, fiberglass tanks (dimensions 1.5 × 1.5 × 0.5 m; water depth 30 cm; water flow 4 L/min/kg fish; n = 3 tanks/ ploidy; approx. 5400 larvae/tank) under continuous light (2 × 18 W fluorescent daylight tubes, OSRAM L 18W/ 840 LUMILUX OSRAM GmbH, Ausburg, Germany) and continuous feeding using automatic disc feeders and a commercial start feed (NUTRA ST 0.5, Skretting AS, Fontaine-les-Vervins, France) that increased in size (up to 1.5 mm pellets, Skretting AS, Norway). On July 22 2010, the fish from each tank were transferred to single, green, fiber glass, 10.7 m 3 tanks (Namdal Plast AS, 3 m Ø; water depth 1 m; n = 3 tanks/ploidy; n = 6 tanks). The fish were reared under continuous light (light source as above) from start-feeding onwards. The mean temperature in the period fertilization to start-feeding was 4.9°C (min 3.2°C, max 6.8°C), and in the period start-feeding to August 2010 it was 13.2°C (min 10.7°C, max 15.6°C). The oxygen saturation in the outlet water was always above 80%.
To determine ploidy status, the diameter of the erythrocytes was measured. This method has previously been validated for identification of triploid salmonids, whose erythrocytes are consistently larger than those in diploids [41]. Blood smears were taken from a total of eighty fish per ploidy (n = 160). The diameters of 10 random red blood cells were measured (Image-Pro Plus, version 4.0, Media Cybernetics Silver Spring, MD, USA) on each blood smear. There was no overlap in mean red blood cell diameters between diploid (mean 16.8 μm, max 18.1 μm) and triploid (mean 20.8 μm, min 19.8 μm) individuals, suggesting 100% efficiency of ploidy manipulation.

Sampling procedure
On Aug 24 2010, twelve juvenile fish (n = 6/ploidy) of unknown sex (mean weight: diploid 25.5 g, triploid 28.0 g; mean length: diploid 112 mm, triploid 118 mm) from six tanks (three tanks/ploidy; two fish/tank) were euthanized by a sharp blow to the head followed by decapitation and sampled for gut microbiology. Sterile latex gloves or latex gloves wiped with 70% ethanol were used throughout the dissection. The underside of the fish was sterilized using 70% ethanol and the ventral belly surface was opened with a sterile surgical blade and forceps. The spleen, gall bladder, liver, and fat deposits surrounding the GI tract were gently removed to expose the peritoneal cavity without disturbing the intestine. The intestine was divided into foregut, midgut, and hindgut as previously described [42]. Thereafter, the intestinal content from within each gut section was squeezed into separate sterile tubes and the appropriate 10-fold dilutions were prepared in physiological saline. Then 100 μl aliquots were pipetted onto the surface of 5% cattle blood agar (blood agar base no 2, Difco) and Brocalin agar (Merck, Darmstadt, Germany) both with 1% NaCl. Dry feed samples and water samples were also appropriately diluted and streaked onto blood agar and Brocalin agar. The plates were incubated at 12°C (± 1) and inspected regularly for up to 4 weeks.

Bacterial isolation, genus identification, and quantification
After incubation, each morphologically distinct colony (form, size, surface, color, texture, elevation, margin, and hemolysis) was coded. To obtain pure growth, two randomly selected representatives of all the coded bacterial colonies from each section of the intestine, were subcultured on blood agar. Afterwards, pure cultured colonies were checked for motility under a light microscope (Leitz, 301-314.001, Germany) at 1000 × magnification using a glass slide with suspended and Gram stained exponentially grown bacteria in physiological salt water [43]. Catalase and oxidase activity were tested as well as the ability to reduce nitrate. The IMViC reactions; indol production, methyl red test (fermentation of glucose), Voges-Proskauer reaction (diacetyl-production) and the ability to use citrate as the sole carbon source (Simmons citrate medium) were tested. H 2 S production, gelatinase, urease and β-galactosidase (ONPG) production and ability to degrade glucose, trehalose, lactose, maltose, mannitol and esculin were tested [ Table 1].
From both fish lines a total of 552 different isolates were identified to the genus level based on morphological and biochemical characteristics and compared with Bergey's Manual of Determinative Bacteriology, Ninth Edition (2000). The total count of named viable bacteria was indicated in terms of colony forming units per mg (cfu/mg).

Antibiotic susceptibility testing of isolated strains
Two identified colonies of Acinetobacter spp., Pseudomonas spp., Pseudomonas fluorescens, Serratia spp., Vibrio spp. and Psychrobacter spp. were randomly selected from each individual fish. All coded bacterial colonies were identified from the hindgut, with the exception of Acinetobacter spp. that was only sampled from the foregut. Antimicrobial susceptibility of a total of 144 isolates was tested against tetracycline (Tet 80 μg), sulphonamide (Sul 240 μg), trimethoprim (Trim 5. GN curved or comma shaped rod (smooth, moist, opaque-white \yellow green, round 2-3 mm colonies) GN regular rods (Shiny, orangeyellow, circular, smooth, concave, 2 mm colonies) GP irregular rods with jointed rods 'V-shape' (Yellow-Greenish-metallic center,1-2 mm colonies) GP straight long-narrow rods in chain, contain oval endospore (Graywhite, dry appearance 3-4 mm) GP slightly slender singly rods (Whitish, round, convex, 1-2 mm colonies) GP cocci singly, in pairs (Circular, convex to slightly peaked, smooth, glossy, opaque 1-5 mm colonies)   [44]. All positions showing differences to the best-scoring reference sequence were visually inspected in the electropherogram, and the sequence was corrected manually if necessary as previously described [45]. Thereafter, a second search was done using BLASTN. Undetermined nucleotides (designated N) in either the determined sequence or the reference sequence were counted as matches. All derived sequences have been submitted to GenBank, with accession numbers listed in [ Table 2].

Statistical analysis GI microflora
The data was recorded in a Microsoft Office Excel 2003 ® spread sheet and then transferred to JMP ® 8.0 Statistical Discovery Software from SAS for statistical analysis. After descriptive analyses of data using standard summary measures and graphical representation, two least-squares models were established, with total bacterial count and individual bacterial species count as the outcomes. First, the effect of ploidy (ordinal, diploid versus triploid) and gut section (ordinal, foregut, midgut and hindgut) on total bacteria count was tested. Secondly, a similar model was established for individual bacterial species. The model gave a standard analysis of variance table and the corresponding regression coefficients. Model fit was assessed using graphical techniques, plotting actual versus predicted values, and residuals were assessed using the normal quantile plot.
To investigate for possible tank effects, parametric and non-parametric tests were used with i) the tank considered the unit of measure (n = 3/ploidy) and ii) the tank included within a nested model design (with individual fish the unit of measure, n = 6/ploidy). No tank effects were observed, with the results (the effect of ploidy on gut microbiota) remaining the same whether tank was included in the model or not (data not shown). Graphs were made in Microsoft Word Excel and JMP ® . Results were considered significant at P < 0.05.

Antibacterial resistance
Antibiotic

Results
Fish growth was not affected by the triploid treatment (data not shown), and all fish appeared healthy with no gross deformities or lesions at the macroscopic level within the gut after visual inspection. Furthermore, by naked eye, there was no discernable difference in the amount of digesta within the gut of individual fish.

GI microflora
On the basis of physiological and biochemical identification 10 different bacterial genera were isolated and characterized within the GI tract of each ploidy and these corresponded to the genetic analysis [Tables 1 and 2]. There was no effect of ploidy on the bacterial diversity of the GI tract, or from which gut section the individual species were isolated [ Figure 1]. No bacteria dominated the bacterial counts either as a percentage of the whole gut, or within specific gut sections, with Pseudomonas spp., P. fluorescens, Pectobacterium carotovorum, Psychrobacter spp., Arthrobacter spp., Staphylococcus spp., Bacillus spp., and Carnobacterium spp., each being between 8 and 13% of the total GI bacterial counts. There was a statistical significance in level of total bacterial counts from the foregut to the hindgut in both ploidy; however triploids had significantly greater total bacterial counts within each gut section compared to diploids [ Figure 2]. Triploids had on average 7.3% more bacteria within the whole gut than diploids (max diploid and min triploid value 102.1 and 105.8 cfu/ml respectively), with the largest increase observed within the foregut (10%). Triploidy had a significant effect on a number of the individual bacterial species isolated [ Table 3]. Significantly increased levels of Pseudomonas sp., Pectobacterium carotovorum, Psychrobacter spp., Bacillus spp., and Vibrio spp., were observed in triploid fish compared to diploids (triploids having on average 12, 42, 9, 10, and 11% more bacteria than diploids, respectively), whereas a decrease in Carnobacterium spp., within triploids compared to diploids was close to significant (P = 0.1, diploids having on average 8% more bacteria than triploids). Where significant increases in bacteria were observed between the ploidies, the increase was not always universal along the whole gut. The increase in Pseudomonas spp., and Vibrio spp., over diploid counterparts was more pronounced in the forgut and midgut respectively, compared to Pectobacterium carotovorum, Psychrobacter spp., and Bacillus spp., where there was a similar increase in triploid bacterial populations along the entire gut length [ Figure 1].

Antibacterial resistance
No ploidy effect was observed on bacterial multiple drug resistance, or the numbers of antibiotics the bacteria were resistant to. For diploid and triploid isolated bacteria respectively, the frequency of resistant bacteria to two antibacterials was 16 and 20.8% with 9.7 and 13.2% of those resistant to more than two antibacterials. All strains were susceptible to gentamicin, whilst the least resistance was demonstrated against chloramphenicol which inhibited more than 95% of the strains.
For all the antibiotics where resistance was observed, there was a general trend of increased resistance in isolates from triploid fish compared to isolates from diploid fish [ Figure 3]. Statistically, the presence of tetracycline

Discussion
To our knowledge, this is the first study into the intestinal microbiota of triploid Atlantic salmon, and the first into any species of triploid fish. The objective was to compare the GI microflora and the antibiotic resistance profiles of the intestinal microflora of Atlantic salmon after chromosome set manipulation (triploidy) to see if it resulted in an altered physiological profile. No differences were observed in the diversity of bacteria isolated from the gut of triploid or diploid fish, however, triploid fish were found to contain greater total bacterial counts within each gut section [ Figure 2], although this was not due to a general increase in all the isolated bacteria [ Table 3]. For specific bacteria, triploids were found to contain significantly more Pseudomonas sp., Pectobacterium carotovorum, Psychrobacter spp., Bacillus spp., and Vibrio spp., whereas Carnobacterium spp. was close to being significantly lower in triploids compared to diploids. These results suggest the physiological differences associated with triploidy have an influence on the gut microbiota. Bacterial culturing and 16S rRNA PCR were used in this study to identify and quantify the bacteria within the fish gut. It is acknowledged that traditional culturing may only identify 11-50% of the present bacteria [46,47]. Additionally, competitive differences between the bacteria on the culture medium compared to the fish gut may lead to misrepresentation of the microbiota community [42], and the media used in this study, blood agar and Brocalin agar, are not traditionally used in fish studies and may not have supported growth of Lactobacillus spp., previously found to be dominant in the GI tract of Atlantic salmon [48]. Metagenomic analysis may have led to a more realistic representation of the GI microflora in this study, however, this method is relatively underdeveloped in Atlantic salmon and not comparable to the existing literature that There was a significant difference (p < 0.05) in colony forming units (CFU) between all gut sections. Compared to diploids, triploid salmon had a statistically significantly (p < 0.05, indicated by an asterisk) greater number of bacteria within each gut section.
uses methods that are still routinely, and historically, used in the field of microbiology. As such, previously identified increases in the level of bacteria from the foregut to the hindgut were observed in this study [23,42], along with the appearance of certain bacteria inhabiting the different gut sections within salmonids [20,42,49]. Therefore, the authors believe the results of this study are reliable for making an accurate comparison related to ploidy within this study.
Differences were observed in the culturable bacteria levels between the triploid and diploid tank water. Increases in Bacillus spp. and Vibrio spp. within triploid tanks were consistent compared to diploid tanks, however the levels of the other isolated bacteria were similar. All tanks were supplied with the same inflow water that contained neither Vibrio spp., nor Bacillus spp., however, feed did contain Bacillus spp. Therefore, increases in Bacillus spp. could be explained by an increase in uneaten feed in the triploid tanks; however, feed intake was not assessed and uneaten feed was removed by the self-cleaning design of the tank, and this would not explain the increase in Vibrio spp. Alternatively, the increases in Vibrio spp. and Bacillus spp., could be explained by the release of allochthonous bacteria that are only transient in the gut and are released over time in faeces. Therefore the increase in Bacillus  Figure 3 The average frequency distributions of antibiotic resistant gram negative isolates (N = 144) among triploid and diploid fish.
There was a trend of increased antibacterial resistance from bacteria isolated from triploids compared to those bacteria isolated from diploids, this was statistically significant (p < 0.05, indicated by an asterisk) for some antibiotics. spp., and Vibrio spp., in triploid compared to diploid tank water could be expected due to the increases in these two bacteria observed within the triploid gut. However, this was not the case for Pseudomonas spp., Pectobacterium carotovorum, and Psychrobacter spp. that were found in equal quantities in tank water from both ploidies, despite being found to have increased numbers within the triploid gut. The reasons for this are unknown, but may be related to the ability of different bacterial species to adhere to the intestinal mucosa as part of the autochthonous flora and therefore not be released in such great quantities in the faeces, or the ability of these bacteria to survive and compete within the tank water. Limitations in our study include no control for the effect of pressure treatment independent of ploidy, and the effect of genetic variation within the study fish. Firstly, the current evidence would suggest that triploid induction procedures (when optimized) do not influence triploid performance [12,13,16], although Malison et al. [50] concluded otherwise, this study failed to have an adequate control. With regards to the genetic composition of the study population, it is recognized family effects are observed on triploid survival and physiological performance [51] and this may influence gut microbiota, however we did not test for this. Therefore, the study should be repeated with an emphasis on the family effect on gut microbiota in fish of both ploidy.
Previously, 10 of the 11 isolated bacteria have been recorded in Atlantic salmon [27,42,52,53]. In addition, the levels of bacteria isolated in this study agree with those previously found in Atlantic salmon [42]. However, we could not find any previous report of Pectobacterium carotovorum subsp. carotovorum, previously isolated from Chinese cabbage [Zhang et al. 2008, unpublished data], in Atlantic salmon. Its source and biological significance in this study are unknown.
Our results suggest the triploid gut provides a different environment to that of the diploid gut, based on bacterial levels. Unfortunately, no literature exists on the triploid gut, or triploid physiology that may affect bacterial growth, therefore some of the major factors that influence gut microbiota cannot be discussed (i.e. gut mucous, physiochemical environment) [52,54,55]. Although no significant differences on weight, length, or condition factor were seen within this study, significant differences were seen in the growth (triploids were heavier and longer), condition factors (triploids had a lower value), and the hepatosomatic index (higher in triploids) in presmolts. Previously, differences in gut microbiota have been identified between fast and slow growing members of fish from the same population [25], and may explain our results. Alternatively, we also observed differences in the leucocyte composition (neutrophilic and B-cells proportions) in the same study fish three weeks after seawater transfer (Fraser et al, in preparation). It is well established that the gut microbiota influences the development of the immune system in mammals [56] and evidence suggests the same occurs in fish [22]. Therefore, it is possible that differences in the immune system related to ploidy (i.e. leucocyte proportions) are influencing the size and composition of the gut microbiota through interactions with mucosal immune cells, or the mechanisms by which the microbiota influences the immune development in fish may be altered by the ploidy status. Cortisol levels and female sex hormone levels have been found to be lower in triploids compared to diploids [18,57,58], and these may also influence the immune system in fish [59][60][61][62][63][64][65]. However, the majority of studies have observed no differences in cortisol [18,[62][63][64][65] due to ploidy and, up until the time of maturing sex hormone levels are typically below detectable levels in salmon and therefore unlikely to influence the immune system in this study. A final explanation is that of differences in cell size and number relating to ploidy. It is well established that cell size increases with increasing numbers of chromosome sets [6]. This phenomenon is more apparent in cells where the nucleus occupies a greater proportion of the total cell volume, so may have little effect on some intestinal cells (i.e. enterocytes). However, triploids have organs of equal sizes to diploids due to reductions in cell number [6,7], and it may be that triploids have fewer cells along the gastrointestinal tract, such as goblet cells, altering the conditions within the gut. Therefore, differences in the ontogenetic growth, the immune system, or gut environment in the triploids may explain our results.
A difference in the gut microbiota between the ploidies could have a number of implications on triploid aquaculture practices. For instance, Vibrio spp., are known to be opportunistic pathogens and causative agents of disease and mass mortalities [26], Pseudomonas spp., Bacillus spp, and Carnobacterium spp., are capable of inhibiting pathogenic bacteria and improving survival in vivo after challenge tests [47,66], Psychrobacter spp., and Arthrobacter agillis have been linked to enteritis in Atlantic salmon [27], and Bacillus spp., and Carnobacterium spp., are commonly used in fish pro-and pre-biotics [23,30]. These results suggest that the susceptibility of triploids to opportunistic pathogens (important in both fish and human health) or potential probiotic treatments may be increased in comparison to diploids. Indeed, several studies have reported triploids to be more prone to disease than diploids [12][13][14][15].
Despite no known antibiotic exposure within our study population, we detected a variety of drug resistance patterns within bacterial isolates. Previous studies have reported antibacterial drug resistance in various aquatic environments [67][68][69] at levels higher than those found within our study, possibly as a result of antibiotic use that exerts an ecological pressure on bacteria [70]. However, increased levels of antibiotic resistant bacteria, over that of the local environment, have been reported in aquaculture facilities that are not utilizing antibiotics [39], and the reasons for this remain unclear.
Triploids were found to have consistently higher levels of antibacterial resistance in their bacterial isolates than in diploids; with 3 of the 6 effective antibacterials significantly affected by ploidy [ Figure 3] but no ploidy effect was seen on the occurrence of multiple drug resistance. We suggest two possible explanations for the first observation i) possible physiological differences between the two ploidies (as discussed above) impacting upon the host-bacterium interaction, and ii) the significantly greater levels of bacteria found within the triploid compared to diploid intestine might provide better conditions for more efficient bacterial colonization, which is very critical for horizontal gene transfer [71]. Unfortunately, both of these hypotheses were outside the scope of this study.

Conclusions
This study would suggest the altered physiology of triploid fish affects the numbers of microbiota within GI tract and their drug resistance profiles. The observation of altered drug resistance profiles between triploid and diploid fish would also make for an interesting follow up study to determine the factors that cause such a phenomenon. It would also be of interest to see how the observed differences in bacterial communities are affected by fish age, genotype, and rearing environment, as these factors are known to alter the microbiota of the GI tract in diploids. It has also been observed that triploids can demonstrate altered growth patterns compared to diploids, such as delayed time of first feeding and slower growth in the juvenile phase, and the effects on/of the GI microbiota may be of interest.