Table 1 Effects of gold nanoparticles, hsp90-1 nanogold conjugated and non nanogold conjugated hsp90-1 on Ichthyophthirius multifiliis. Mean of 6 wells each test (± SD) numbers 2 and 12 h post exposure to different concentrations of treatment solutions
Solution | Solution concentrat ion (ng mL− 1) | Mean number of killed trophonts/well after 2 h (each well started with 25 trophonts) +/- SD | Mean % dead trophonts after 2 h | Mean number of killed tomocysts/ well after 2 h (each well started with 15) +/- SD | Mean % dead tomocysts after 2 h | Mean number of killed theronts/ well after 2 h (each well started With ~ 75) +/- SD | Mean % dead theronts after 2 h | Mean number of killed trophonts/ well after 12 h (each well started with 25 trophonts) +/- SD | Mean % dead trophontts after 12 h | Mean number of killed tomocysts/ well after 12 h (each well started with 15 tomocysts) +/- SD | Mean % dead tomocysts after 12 h | Mean number of killed theronts/well after 12 h (each well started with ~ 75 theronts) +/- SD | Mean % dead theronts after 12 h |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Gold nano particles | 0 2.5 5 10 20 40 80 160 | 2 ± 0.3 5 ± 1.7 7 ± 1.1 10 ± 1.0 12 ± 1.3 12 ± 1.9 12 ± 1.1 12 ± 1.2 | 8% 20% 28% 40% 48% 48% 48% 48% | 0 3 ± 1.7 4 ± 1.1 5 ± 1.9 7 ± 1.2 7 ± 1.3 7 ± 1.6 7 ± 1.4 | 0% 20% 26.7% 33.3% 46.7% 46.7% 46.7% 46.7% | 0 15 ± 1.7 23 ± 1.1 30 ± 1.9 37 ± 1.1 37 ± 1.1 37 ± 1.2 37 ± 1.3 | 0% 20.7% 31.3% 40.7% 49.3% 49.3% 49.3% 49.3% | 2 ± 1.3 6 ± 1.7 11 ± 1.1 15 ± 1.9 20 ± 1.3 20 ± 1.1 20 ± 1.5 20 ± 1.3 | 8% 24% 44% 60% 80% 80% 80% 80% | 0 6 ± 1.9 8 ± 1.3 10 ± 1.5 12 ± 1.1 12 ± 1.9 12 ± 1.6 12 ± 1.7 | 0% 40% 53.3% 66.7% 80% 80% 80% 80% | 0 20 ± 1.1 25 ± 1.7 50 ± 1.9 60 ± 1.9 60 ± 1.3 60 ± 1.9 60 ± 1.2 | 0% 26.7% 33.3% 66.7% 80.8% 80.8% 80.8% 80.8% |
Hsp90-1 nanogold conjugated | 0 2.5 5 10 20 40 80 160 | 2 ± 1.3 8 ± 1.6 10 ± 1.1 13 ± 1.6 13 ± 1.4 13 ± 1.5 13 ± 1.3 13 ± 1.4 | 8% 30% 40% 52% 52% 52% 52% 52% | 0 7 ± 1.6 7 ± 1.1 8 ± 1.9 8 ± 1.5 8 ± 1.8 8 ± 1.6 8 ± 1.1 | 0% 46.7% 46.7% 53.3% 53.3% 53.3% 53.3% 53.3% | 0 28 ± 1.7 30 ± 1.9 39 ± 1.7 39 ± 1.5 39 ± 2.1 39 ± 2.2 39 ± 1.1 | 0% 38% 40% 52% 52% 52% 52% 52% | 2 ± 1.3 13 ± 12 14 ± 1.5 25 ± 1.9 25 ± 1.5 25 ± 1.7 25 ± 1.6 25 ± 1.9 | 8% 46% 58% 100% 100% 100% 100% 100% | 0 8 ± 1.6 10 ± 1.3 15 ± 1.6 15 ± 1.7 15 ± 1.9 15 ± 1.7 15 ± 1.4 | 0% 56.7% 70% 100% 100% 100% 100% 100% | 0 55 ± 1.7 69 ± 1.9 75 ± 1.1 75 ± 1.3 75 ± 1.9 75 ± 1.1 75 ± 1.9 | 0% 73.3% 92% 100% 100% 100% 100% 100% |
Hsp90-1 non nanogold conjugated | 0 2.5 5 10 20 40 80 160 | 2 ± 1.3 11 ± 1.6 11 ± 1.8 12 ± 1.6 13 ± 1.3 13 ± 1.4 13 ± 1.7 13 ± 1.7 | 8% 42% 42% 48% 52% 52% 52% 52% | 0 4 ± 1.1 6 ± 1.5 7 ± 1.9 8 ± 1.2 8 ± 1.3 8 ± 1.4 8 ± 1.2 | 0% 26.7% 40% 46.7% 53.3% 53.3% 53.3% 53.3% | 0 55 ± 1.2 35 ± 1.5 37 ± 1.9 38 ± 1.3 38 ± 1.7 38 ± 1.5 38 ± 1.9 | 0% 36.7% 46.7% 49.3% 50.7% 50.7% 50.7% 50.7% | 2 ± 1.7 15 ± 1.9 15 ± 1.1 25 ± 1.4 25 ± 1.3 25 ± 1.1 25 ± 1.3 25 ± 1.7 | 8% 70% 70% 100% 100% 100% 100% 100% | 0 9 ± 1.3 10 ± 1.7 15 ± 1.9 15 ± 1.5 15 ± 2.1 15 ± 2.2 15 ± 1.7 | 0% 60% 66.7% 100% 100% 100% 100% 100% | 0 50 ± 1.2 55 ± 1.5 70 ± 1.9 70 ± 1.7 70 ± 1.9 70 ± 1.5 70 ± 1.5 | 0 66.7% 73.3% 93.3% 93.3% 93.3% 93.3% 93.3% |