Fig. 4
Selection of the mAb9 for ELISA development. A The purified anti-S mAbs were analyzed by SDS-PAGE. The original gel is presented in Additional file 2. B Selection of the optimal anti-S mAb for developing ELISA. Each purified anti-S mAb was two-fold serially diluted as 8, 4, 2, 1, 0.5, 0.25, 0.125 and 0.0625 μg/mL, and coated on 96-well ELISA plates. Then, the plates were blocked and incubated with inactivated cell-cultivated PEDV supernatants (1 mg/mL). The plates were incubated with the rabbit anti-PEDV pAbs and HRP-conjugated goat anti-rabbit IgG, and reacted with TMB substrate solution. After stopped, OD450 values were measured on the BMG-Labtech microplate reader. The error bars indicate SD values