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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Development of an enzyme-linked immunosorbent assay based on viral antigen capture by anti-spike glycoprotein monoclonal antibody for detecting immunoglobulin A antibodies against porcine epidemic diarrhea virus in milk

Fig. 3

IFA analyses of PEDV-infected Vero cells recognized by the anti-S mAbs. The Vero cells with the 80% confluence were inoculated with PEDV until exhibiting obvious CPEs. After fixed and blocked, the cells were incubated with the anti-S mAbs and Alexa Fluor 488-donkey anti-mouse IgG. The cell nuclei were stained using DAPI and the cells were visualized by Leica fluorescence microscopy. Representative images were shown. Scale bars, 200 μm

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