Skip to main content
Fig. 2 | BMC Veterinary Research

Fig. 2

From: Development of an enzyme-linked immunosorbent assay based on viral antigen capture by anti-spike glycoprotein monoclonal antibody for detecting immunoglobulin A antibodies against porcine epidemic diarrhea virus in milk

Fig. 2

Identification of anti-S mAbs by Western blot. A The recombinant PEDV S1 subunit was purified by HisTrap excel prepacked column and HiTrap Q HP prepacked column, and then analyzed by SDS-PAGE. B The purified PEDV S1 subunit was applied to SDS-PAGE, transferred to PVDF membranes, and detected by the mAbs 9, 10, 17 and 18. In parallel, mock control and recombinant TGEV S protein were applied. The results were visualized with HRP-conjugated goat anti-mouse IgG and Solarbio ECL plus reagent. The original gel and blots are presented in Additional file 2

Back to article page