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Table 2 Numerical results of 3 ELISAs (optical density, OD) used in the study presented as the arithmetic mean ± standard deviation and range in parentheses

From: Serological testing of an equal-volume milk sample – a new method to estimate the seroprevalence of small ruminant lentivirus infection?

ELISAa

Seronegative goats (n = 100)

Seropositive goats (n = 100)

serum

milk

serum

milk

Raw OD

Corrected ODb [%]

Raw OD

Corrected ODb [%]

Raw OD

Corrected ODb [%]

Raw OD

Corrected ODb [%]

sp-iELISA

0.073 ± 0.038 (0.048 – 0.297)

2.3 ± 3.6 (-0.3 – 20)

0.065 ± 0.028 (0.045 – 0.240)

1.5 ± 2.5 (-0.5 – 15)

4.019 ± 0.248 (3.061 – 4.2)

353 ± 34 (286 – 429)

3.625 ± 0.690 (1.159 – 4.2)

316 ± 59 (111 – 403)

TM/CA-iELISA

0.160 ± 0.045 (0.100 – 0.330)

8.2 ± 4.8 (1.8 – 20)

0.169 ± 0.122 (0.051 – 0.933)

9.2 ± 12 (-2.7 – 78)

3.934 ± 0.297 (2.924 – 4.2)

376 ± 48 (302 – 474)

3.887 ± 0.443 (1.926 – 4.2)

372 ± 63 (179 – 474)

SU-cELISA

0.810 ± 0.156 (0.517 – 1.385)

-1.3 ± 12 (-45 – 9.9)

0.737 ± 0.179 (0.275 – 1.596)

7.6 ± 18 (-56 – 67)

0.189 ± 0.085 (0.062 – 0.383)

74 ± 12 (52 – 93)

0.286 ± 0.120 (0.059 – 0.585)

61 ± 15 (28 – 93)

  1. a ELISA used: sp-iELISA – indirect ELISA based on a mixture of synthetic viral peptides, TM/CA-iELISA – indirect ELISA based on recombinant transmembrane and capsid proteins, and SU-cELISA – competitive ELISA based on surface glycoprotein antigen
  2. b corrected optical density: sp-iELISA and TM/CA-iELISA – serum-to-positive control ratio (S/P%), SU-cELISA – percentage of inhibition (PI)
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BMC Veterinary Research

ISSN: 1746-6148

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